Cloned (Comment) | Organism |
---|---|
expression of His-tagged nitrate reductase in Escherichia coli | Synechococcus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azide | competitive inhibition | Synechococcus sp. | |
cyanide | mixed inhibition, 50% inhibition at 0.01 mM | Synechococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
nitrate | 25°C, pH 8.0, electron donor methylviologen | Synechococcus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | nitrate reductase contains 1 [4Fe-4S] cluster | Synechococcus sp. | |
Molybdenum | - |
Synechococcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78000 | - |
x * 78000, SDS-PAGE | Synechococcus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrate + reduced ferredoxin | Synechococcus sp. | - |
nitrite + H2O + oxidized ferredoxin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechococcus sp. | - |
PCC 7942 | - |
Purification (Comment) | Organism |
---|---|
recombinant nitrate reductase | Synechococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorate + reduced methylviologen | 6% of kcat with nitrate | Synechococcus sp. | chlorite + H2O + oxidized methylviologen | - |
? | |
nitrate + reduced ferredoxin | - |
Synechococcus sp. | nitrite + H2O + oxidized ferredoxin | - |
? | |
nitrate + reduced methylviologen | - |
Synechococcus sp. | nitrite + H2O + oxidized methylviologen | - |
? | |
selenate + reduced methylviologen | 1% of kcat with nitrate | Synechococcus sp. | selenite + H2O + oxidized methylviologen | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 78000, SDS-PAGE | Synechococcus sp. |
Synonyms | Comment | Organism |
---|---|---|
narB | - |
Synechococcus sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
80 | - |
nitrate | 25°C, pH 8.0, electron donor methylviologen | Synechococcus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
molybdopterin | - |
Synechococcus sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
13 | - |
azide | 25°C, pH 8.0 | Synechococcus sp. |