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Literature summary for 1.7.7.2 extracted from

  • Lledo, B.; Martinez-Espinosa, R.M.; Marhuenda-Egea, F.C.; Bonete, M.J.
    Respiratory nitrate reductase from haloarchaeon Haloferax mediterranei: biochemical and genetic analysis (2004), Biochim. Biophys. Acta, 1674, 50-59.
    View publication on PubMed
Search for more literature for 1.7.7.2

Inhibitors

Inhibitors Comment Organism Structure
azide 1 mM, 68% loss of activity Haloferax mediterranei
cyanide 1 mM, 76% loss of activity Haloferax mediterranei
Dithionite 1 mM, 24% loss of activity Haloferax mediterranei
EDTA 1 mM, 22% loss of activity Haloferax mediterranei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
 methyl viologen
-
 Haloferax mediterranei
0.82
-
 nitrate
-
 Haloferax mediterranei

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Haloferax mediterranei 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Iron enzyme contains Fe-S clusters. NarB codes for a 219-amino-acid-residue iron Rieske protein. Haloferax mediterranei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Haloferax mediterranei the regulation of the nar gene occurs at transcriptional level induced by oxygen-limiting conditions and the presence of nitrate ?
-
 ?

Organism

Organism UniProt Comment Textmining
Haloferax mediterranei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Haloferax mediterranei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Haloferax mediterranei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the regulation of the nar gene occurs at transcriptional level induced by oxygen-limiting conditions and the presence of nitrate Haloferax mediterranei ?
-
 ?
nitrate + reduced methyl viologen reduced methyl viologen is the best electron donor. NADPH or NADH show only 2.4% of maximal activity, no activity with FADH2 Haloferax mediterranei nitrite + methyl viologen
-
 ?

Synonyms

Synonyms Comment Organism
respiratory nitrate reductase
-
 Haloferax mediterranei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 at 3.4 M NaCl Haloferax mediterranei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
 at 40°C Haloferax mediterranei
8.2
-
 at 60°C Haloferax mediterranei