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Literature summary for 1.7.7.2 extracted from

  • Hirasawa, M.; Rubio, L.M.; Griffin, J.L.; Flores, E.; Herrero, A.; Li, J.; Kim, S.K.; Hurley, J.K.; Tollin, G.; Knaff, D.B.
    Complex formation between ferredoxin and Synechococcus ferredoxin: nitrate oxidoreductase (2004), Biochim. Biophys. Acta, 1608, 155-162.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of native and His-tagged nitrate reductase in Escherichia coli Synechococcus sp.

Inhibitors

Inhibitors Comment Organism Structure
KCl inhibition of nitrate reductase-ferredoxin complex formation at high ionic strength Synechococcus sp.
MgSO4 inhibition of nitrate reductase-ferredoxin complex formation at high ionic strength Synechococcus sp.
N-acetylsuccinimide 115fold excess, loss of more than 80% and 5-10% loss of ferredoxin-dependent and methylviologen dependent activity after 1 h, respectively Synechococcus sp.
NaCl more than 80% inhibition above 200 mM, inhibition of nitrate reductase-ferredoxin complex formation at high ionic strength Synechococcus sp.
Phenylglyoxal 76fold excess, approx. 60% and 20% loss of ferredoxin dependent and methylviologen dependent activity after 2 h, respectively Synechococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
oxidized ferredoxin spinach ferredoxin Synechococcus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Iron enzyme contains one iron sulfur cluster Synechococcus sp.
Molybdenum enzyme probably contains a bis-molybdopterin guanine dinucleotide Synechococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
76000
-
x * 76000, deduced from nucleotide sequence Synechococcus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nitrate + reduced ferredoxin Synechococcus sp.
-
nitrite + H2O + oxidized ferredoxin
-
?

Organism

Organism UniProt Comment Textmining
Synechococcus sp.
-
PCC 7942
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged nitrate reductase, Ni2+-affinity column Synechococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitrate + reduced ferredoxin
-
Synechococcus sp. nitrite + H2O + oxidized ferredoxin
-
?
nitrate + reduced ferredoxin spinach ferredoxin or Anabaena ferredoxin Synechococcus sp. nitrite + H2O + oxidized ferredoxin
-
?
nitrate + reduced methylviologen
-
Synechococcus sp. nitrite + H2O + oxidized methylviologen
-
?
nitrite + H2O + oxidized ferredoxin
-
Synechococcus sp. nitrate + reduced ferredoxin + H+
-
r

Subunits

Subunits Comment Organism
? x * 76000, deduced from nucleotide sequence Synechococcus sp.

Cofactor

Cofactor Comment Organism Structure
molybdopterin
-
Synechococcus sp.