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Literature summary for 1.7.7.2 extracted from
- Studies on nitrate reductase of Clostridium perfringens. Purification, some properties, and effect of tungstate on its formation (1977), J. Biochem., 82, 1663-1671.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | enzyme synthesis is induced by nitrate | Clostridium perfringens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| azide | 74% inhibition | Clostridium perfringens |  |
| cyanide | 10 mM, 87% inhibition | Clostridium perfringens | |
| additional information | not inhibited by 0.1 mM p-chloromercuribenzoate, enzyme formation is induced by nitrate and inhibited by 0.5 mM tungstate, but recovered by 0.1 mM molybdate | Clostridium perfringens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
nitrate | - |
Clostridium perfringens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | enzyme functions in the soluble state in the cytoplasm | Clostridium perfringens | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | stimulates activity about 30% | Clostridium perfringens | |
| Fe2+ | stimulates activity about 50% | Clostridium perfringens | |
| Iron | molybdenum iron-sulfur protein | Clostridium perfringens | |
| iron-sulfur centre | molybdo-iron-sulfur protein | Clostridium perfringens | |
| Mg2+ | stimulates activity about 30% | Clostridium perfringens | |
| Mn2+ | stimulates activity about 50%, stimulates reaction rate and level of nitrite formation | Clostridium perfringens | |
| Molybdenum | molybdenum iron-sulfur protein | Clostridium perfringens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
gel filtration | Clostridium perfringens |
| 90000 | - |
ultracentrifugation | Clostridium perfringens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nitrate + reduced ferredoxin | Clostridium perfringens | nitrate fermentation, ferredoxin is utilized as direct electron donor in nitrate reduction | nitrite + oxidized ferredoxin | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium perfringens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Clostridium perfringens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 52 | - |
- |
Clostridium perfringens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | not as electron donors: NADH, NADPH, FADH2, FMNH2, rubredoxin | Clostridium perfringens | ? | - |
? | |
| nitrate + reduced ferredoxin | ferredoxin as electron donor at neutral pH and pH 8, but not at pH 9 | Clostridium perfringens | nitrite + H2O + oxidized ferredoxin | - |
? | |
| nitrate + reduced ferredoxin | nitrate fermentation, ferredoxin is utilized as direct electron donor in nitrate reduction | Clostridium perfringens | nitrite + oxidized ferredoxin | - |
? | |
| nitrate + reduced methyl viologen | nitrite formation 10times higher at pH 9 than at pH 8 | Clostridium perfringens | nitrite + oxidized methyl viologen | - |
? | |
| nitrate + reduced methyl viologen | enzyme uses also reduced methyl viologen as reductant | Clostridium perfringens | nitrite + oxidized methyl viologen | - |
? | |
| nitrate + reduced methyl viologen | enzyme also has methyl viologen-linked activity | Clostridium perfringens | nitrite + oxidized methyl viologen | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 90000, SDS-PAGE | Clostridium perfringens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Clostridium perfringens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
reduced methyl viologen as electron donor | Clostridium perfringens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8.3 | 9.8 | at pH 8.3 and 9.8: about 50% of activity maximum | Clostridium perfringens |
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