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Literature summary for 1.7.7.2 extracted from

  • Mikami, B.; Ida, S.
    Purification and properties of ferredoxin-nitrate reductase from the cyanobacterium Plectonema boryanum (1984), Biochim. Biophys. Acta, 791, 294-304.
No PubMed abstract available

General Stability

General Stability Organism
low ionic strength, 70 h, 0.002 mg protein/ml, 10 mM phosphate buffer or 50 mM Tris-HCl buffer, pH 7.5, unstable Leptolyngbya boryana

Inhibitors

Inhibitors Comment Organism Structure
Dithionite converts native enzyme in modified form without ferredoxin-linked activity but retained methyl viologen-linked activity Leptolyngbya boryana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.038
-
reduced ferredoxin
-
Leptolyngbya boryana
2.5
-
reduced methyl viologen
-
Leptolyngbya boryana

Metals/Ions

Metals/Ions Comment Organism Structure
Iron molybdenum iron-sulfur protein Leptolyngbya boryana
Iron contains 4 iron and 4 acid-labile sulfur atoms per enzyme molecule Leptolyngbya boryana
iron-sulfur centre contains 4 iron and 4 acid-labile sulfur atoms per enzyme molecule, presence of iron-sulfur cluster Leptolyngbya boryana
Molybdenum molybdenum iron-sulfur protein Leptolyngbya boryana
Molybdenum enzyme contains 0.95 atom of molybdenum per enzyme molecule Leptolyngbya boryana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
83000
-
1 * 83000, SDS-PAGE Leptolyngbya boryana
85000
-
sedimentation equilibrium method Leptolyngbya boryana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nitrate + reduced ferredoxin Leptolyngbya boryana assimilatory nitrate reduction nitrite + oxidized ferredoxin
-
?
nitrate + reduced ferredoxin Leptolyngbya boryana two-electron nitrate reduction nitrite + oxidized ferredoxin
-
?

Organism

Organism UniProt Comment Textmining
Leptolyngbya boryana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
19000fold purification Leptolyngbya boryana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
305
-
ferredoxin-linked activity Leptolyngbya boryana
1020
-
methyl viologen-linked activity Leptolyngbya boryana

Storage Stability

Storage Stability Organism
-20°C, in 50 mM Tris-HCl buffer, pH 7.5, 0.5 M NaCl, 1 mM EDTA, 1 month, no loss of activity Leptolyngbya boryana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitrate + reduced ferredoxin
-
Leptolyngbya boryana nitrite + H2O + oxidized ferredoxin
-
?
nitrate + reduced ferredoxin assimilatory nitrate reduction Leptolyngbya boryana nitrite + oxidized ferredoxin
-
?
nitrate + reduced ferredoxin two-electron nitrate reduction Leptolyngbya boryana nitrite + oxidized ferredoxin
-
?
nitrate + reduced methyl viologen enzyme also has methyl viologen-linked activity Leptolyngbya boryana nitrite + oxidized methyl viologen
-
?

Subunits

Subunits Comment Organism
monomer 1 * 83000, SDS-PAGE Leptolyngbya boryana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Leptolyngbya boryana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
anaerobic assay, methyl viologen- and ferredoxin-linked activity Leptolyngbya boryana
10.2
-
aerobic assay, methyl viologen-linked activity Leptolyngbya boryana

pH Range

pH Minimum pH Maximum Comment Organism
7.3 9.2 at pH 7.3 and 9.2: 50% of activity maximum, aerobic assay, methyl viologen-linked and ferredoxin-linked activity Leptolyngbya boryana

Cofactor

Cofactor Comment Organism Structure
iron-sulfur centre contains 4 iron and 4 acid-labile sulfur atoms per enzyme molecule, presence of iron-sulfur cluster Leptolyngbya boryana