Any feedback?
Literature summary for 1.7.7.1 extracted from
- Purification and characterisation of a possible assimilatory nitrite reductase from the halophile archaeon Haloferax mediterranei (2001), FEMS Microbiol. Lett., 196, 113-118.
General Stability
| General Stability | Organism |
|---|---|
| the stability of the enzyme depends on the salt concentration. The maximum stability is found in the presence of 3.6 M NaCl | Haloferax mediterranei |
| when the enzyme is dialysed against a 50 mM phosphate buffer, pH 7.0, containing 20% glycerol, the enzyme can be stored for 2 weeks without any significant loss of activity | Haloferax mediterranei |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-hydroxymercuribenzoate | - |
Haloferax mediterranei |  |
| azide | - |
Haloferax mediterranei | |
| cyanide | - |
Haloferax mediterranei | |
| additional information | potassium chlorate and dithiothreitol are not effective inhibitors | Haloferax mediterranei | |
| sulfite | - |
Haloferax mediterranei | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.9 | - |
reduced methyl viologen | pH 7.0, 60°C | Haloferax mediterranei | |
| 8.6 | - |
nitrite | pH 7.0, 60°C | Haloferax mediterranei | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66000 | - |
1 * 66000 | Haloferax mediterranei |
| 74000 | - |
gel filtration | Haloferax mediterranei |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nitrite + 6 reduced ferredoxin + 7 H+ | Haloferax mediterranei | assimilatory nitrite reductase | NH3 + 2 H2O + 6 oxidized ferredoxin | - |
? | |
| nitrite + 6 reduced ferredoxin + 7 H+ | Haloferax mediterranei DSM 1411 | assimilatory nitrite reductase | NH3 + 2 H2O + 6 oxidized ferredoxin | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Haloferax mediterranei | - |
- |
- |
| Haloferax mediterranei DSM 1411 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Haloferax mediterranei |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 15.8 | - |
pH 7.0, 60°C | Haloferax mediterranei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no activity with NADH as electron donor | Haloferax mediterranei | ? | - |
? | |
| additional information | no activity with NADH as electron donor | Haloferax mediterranei DSM 1411 | ? | - |
? | |
| nitrite + 6 reduced ferredoxin + 7 H+ | assimilatory nitrite reductase | Haloferax mediterranei | NH3 + 2 H2O + 6 oxidized ferredoxin | - |
? | |
| nitrite + 6 reduced ferredoxin + 7 H+ | ferredoxin is almost as efficient as methyl viologen as electron donor | Haloferax mediterranei | NH3 + 2 H2O + 6 oxidized ferredoxin | - |
? | |
| nitrite + 6 reduced ferredoxin + 7 H+ | assimilatory nitrite reductase | Haloferax mediterranei DSM 1411 | NH3 + 2 H2O + 6 oxidized ferredoxin | - |
? | |
| nitrite + 6 reduced ferredoxin + 7 H+ | ferredoxin is almost as efficient as methyl viologen as electron donor | Haloferax mediterranei DSM 1411 | NH3 + 2 H2O + 6 oxidized ferredoxin | - |
? | |
| nitrite + FADH2 | 50% of maximum activity compared to reduced methyl viologen as electron donor | Haloferax mediterranei | NH3 + FAD + H2O | - |
? | |
| nitrite + FADH2 | 50% of maximum activity compared to reduced methyl viologen as electron donor | Haloferax mediterranei DSM 1411 | NH3 + FAD + H2O | - |
? | |
| nitrite + NADPH + H+ | 10% of maximum activity compared to reduced methyl viologen as electron donor | Haloferax mediterranei | NH3 + NADP+ + H2O | - |
? | |
| nitrite + reduced 2,6-dichlorophenolindophenol | less than 35% of maximum activity compared to reduced methyl viologen as electron donor | Haloferax mediterranei | NH3 + oxidized 2,6-dichlorophenolindophenol + H2O | - |
? | |
| nitrite + reduced methyl viologen | ferredoxin is almost as efficient as methyl viologen as electron donor | Haloferax mediterranei | NH3 + H2O + oxidized methyl viologen | - |
? | |
| nitrite + reduced methyl viologen | ferredoxin is almost as efficient as methyl viologen as electron donor | Haloferax mediterranei DSM 1411 | NH3 + H2O + oxidized methyl viologen | - |
? | |
| nitrite + reduced phenazine methosulphate | less than 35% of maximum activity compared to reduced methyl viologen as electron donor | Haloferax mediterranei | NH3 + oxidized phenazine methosulphate + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 66000 | Haloferax mediterranei |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| assimilatory NiR | - |
Haloferax mediterranei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
when measured in the presence of 3.3 M NaCl | Haloferax mediterranei |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
at 40°C | Haloferax mediterranei |
| 7.5 | - |
at 60°C | Haloferax mediterranei |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | assimilatory nitrite reductase | Haloferax mediterranei |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
