Literature summary for 1.7.7.1 extracted from

Sakakibara, Y.; Kimura, H.; Iwamura, A.; Saitoh, T.; Ikegami, T.; Kurisu, G.; Hase, T.
A new structural insight into differential interaction of cyanobacterial and plant ferredoxins with nitrite reductase as revealed by NMR and X-ray crystallographic studies (2012), J. Biochem., 151, 483-492.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Synechocystis sp.

Crystallization (Commentary)

Crystallization (Comment)	Organism
NMR-study of protein-protein interaction of ferredoxin and nitrite reductase shows three acidic regions of ferredoxin to be major sites for the interaction with the enzyme, indicating that the complex is stabilized through electrostatic interaction	Synechocystis sp.

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0077	-	reduced ferredoxin	substrate Leptolyngbya boryana ferredoxin mutant E94Q/E95Q, pH 7.5, 25°C	Synechocystis sp.
0.0097	-	reduced ferredoxin	substrate Leptolyngbya boryana ferredoxin, pH 7.5, 25°C	Synechocystis sp.
0.0213	-	reduced ferredoxin	substrate Leptolyngbya boryana ferredoxin mutant D67N/D68N/D69N, pH 7.5, 25°C	Synechocystis sp.
0.0478	-	reduced ferredoxin	substrate Leptolyngbya boryana ferredoxin mutant E31Q/E32Q/D36N, pH 7.5, 25°C	Synechocystis sp.
0.0746	-	reduced ferredoxin	substrate maize ferredoxin, pH 7.5, 25°C	Synechocystis sp.

Organism

Organism	UniProt	Comment	Textmining
Synechocystis sp.	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
nitrite + 6 reduced ferredoxin	-	Synechocystis sp.	ammonia + 2 H2O + 6 oxidized ferredoxin	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
101.4	-	reduced ferredoxin	substrate Leptolyngbya boryana ferredoxin mutant E94Q/E95Q, pH 7.5, 25°C	Synechocystis sp.
138.2	-	reduced ferredoxin	substrate Leptolyngbya boryana ferredoxin, pH 7.5, 25°C	Synechocystis sp.
178.3	-	reduced ferredoxin	substrate Leptolyngbya boryana ferredoxin mutant D67N/D68N/D69N, pH 7.5, 25°C	Synechocystis sp.
203.2	-	reduced ferredoxin	substrate Leptolyngbya boryana ferredoxin mutant E31Q/E32Q/D36N, pH 7.5, 25°C	Synechocystis sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
4300	-	reduced ferredoxin	substrate Leptolyngbya boryana ferredoxin mutant E31Q/E32Q/D36N, pH 7.5, 25°C	Synechocystis sp.
8400	-	reduced ferredoxin	substrate Leptolyngbya boryana ferredoxin mutant D67N/D68N/D69N, pH 7.5, 25°C	Synechocystis sp.
13200	-	reduced ferredoxin	substrate Leptolyngbya boryana ferredoxin mutant E94Q/E95Q, pH 7.5, 25°C	Synechocystis sp.
14200	-	reduced ferredoxin	substrate Leptolyngbya boryana ferredoxin, pH 7.5, 25°C	Synechocystis sp.

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Release 2023.1 (January 2023)