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Literature summary for 1.7.7.1 extracted from
- Purification and characterization of assimilatory nitrite reductase from Candida utilis (1996), Biochem. J., 317, 147-155.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.069 | - |
NADPH | - |
Cyberlindnera jadinii |  |
| 0.08 | - |
methyl viologen | - |
Cyberlindnera jadinii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
1 * 58000 + 1 * 66000, SDS-PAGE | Cyberlindnera jadinii |
| 66000 | - |
1 * 58000 + 1 * 66000, SDS-PAGE | Cyberlindnera jadinii |
| 126000 | - |
gel filtration, native enzyme | Cyberlindnera jadinii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cyberlindnera jadinii | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | 66 kDa subunit is phosphorylated | Cyberlindnera jadinii |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| homogeneity | Cyberlindnera jadinii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 16.06 | - |
- |
Cyberlindnera jadinii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nitrite + NADPH + H+ | - |
Cyberlindnera jadinii | NH3 + NADP+ + H2O | - |
r | |
| nitrite + reduced ferredoxin | NADPH | Cyberlindnera jadinii | ammonia + oxidized ferredoxin | - |
? | |
| nitrite + reduced ferredoxin | other electron donor: methyl viologen | Cyberlindnera jadinii | ammonia + oxidized ferredoxin | - |
? | |
| nitrite + reduced methyl viologen | - |
Cyberlindnera jadinii | NH3 + H2O + oxidized methyl viologen | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 58000 + 1 * 66000, SDS-PAGE | Cyberlindnera jadinii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Cyberlindnera jadinii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
phosphate buffer | Cyberlindnera jadinii |
| 8 | - |
Tris buffer | Cyberlindnera jadinii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | 8.3 | appreciable amounts of activity at pH 7.8 and pH 8.3 in Tris buffer | Cyberlindnera jadinii |
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