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Literature summary for 1.7.7.1 extracted from
- Ferredoxin-nitrite reductase (1980), Methods Enzymol., 69, 255-270.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| repetitive freezing and thawing or prolonged dialysis produces a loss of enzyme activity | Spinacia oleracea |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Synechococcus elongatus PCC 7942 = FACHB-805 | 9507 | - |
| chloroplast | - |
Spinacia oleracea | 9507 | - |
| chloroplast | - |
[Chlorella] fusca | 9507 | - |
| chloroplast | - |
Cucurbita pepo | 9507 | - |
| chloroplast | - |
Anabaena cylindrica | 9507 | - |
| chloroplast | - |
Dunaliella tertiolecta | 9507 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | - |
[Chlorella] fusca |  |
| Iron | - |
Cucurbita pepo | |
| Iron | - |
Dunaliella tertiolecta | |
| Iron | enzyme contains 1 tetranuclear center (4Fe-4S) and 1 siroheme, minimum of 5 Fe atoms per active enzyme molecule | Spinacia oleracea | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nitrite + reduced ferredoxin | Synechococcus elongatus PCC 7942 = FACHB-805 | second enzyme of photosynthetic nitrate-reducing system | ammonia + oxidized ferredoxin | - |
? | |
| nitrite + reduced ferredoxin | Spinacia oleracea | second enzyme of photosynthetic nitrate-reducing system | ammonia + oxidized ferredoxin | - |
? | |
| nitrite + reduced ferredoxin | [Chlorella] fusca | second enzyme of photosynthetic nitrate-reducing system | ammonia + oxidized ferredoxin | - |
? | |
| nitrite + reduced ferredoxin | Cucurbita pepo | second enzyme of photosynthetic nitrate-reducing system | ammonia + oxidized ferredoxin | - |
? | |
| nitrite + reduced ferredoxin | Anabaena cylindrica | second enzyme of photosynthetic nitrate-reducing system | ammonia + oxidized ferredoxin | - |
? | |
| nitrite + reduced ferredoxin | Dunaliella tertiolecta | second enzyme of photosynthetic nitrate-reducing system | ammonia + oxidized ferredoxin | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Anabaena cylindrica | - |
- |
- |
| Cucurbita pepo | - |
- |
- |
| Dunaliella tertiolecta | - |
- |
- |
| Spinacia oleracea | - |
- |
- |
| Synechococcus elongatus PCC 7942 = FACHB-805 | - |
- |
- |
| [Chlorella] fusca | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| homogeneity | Spinacia oleracea |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| NH3 + 2 H2O + 3 oxidized ferredoxin = nitrite + 3 reduced ferredoxin + 7 H+ | mechanism | Synechococcus elongatus PCC 7942 = FACHB-805 | |
| NH3 + 2 H2O + 3 oxidized ferredoxin = nitrite + 3 reduced ferredoxin + 7 H+ | mechanism | Spinacia oleracea | |
| NH3 + 2 H2O + 3 oxidized ferredoxin = nitrite + 3 reduced ferredoxin + 7 H+ | mechanism | [Chlorella] fusca | |
| NH3 + 2 H2O + 3 oxidized ferredoxin = nitrite + 3 reduced ferredoxin + 7 H+ | mechanism | Cucurbita pepo | |
| NH3 + 2 H2O + 3 oxidized ferredoxin = nitrite + 3 reduced ferredoxin + 7 H+ | mechanism | Anabaena cylindrica | |
| NH3 + 2 H2O + 3 oxidized ferredoxin = nitrite + 3 reduced ferredoxin + 7 H+ | mechanism | Dunaliella tertiolecta |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Spinacia oleracea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 108 | - |
- |
Spinacia oleracea |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 50% glycerol, for at least 6 months | Spinacia oleracea |
| 4°C, 0.1 M potassium phosphate buffer, pH 7.7, 1 week | Spinacia oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nitrite + reduced ferredoxin | - |
Synechococcus elongatus PCC 7942 = FACHB-805 | ammonia + oxidized ferredoxin | - |
? | |
| nitrite + reduced ferredoxin | - |
Spinacia oleracea | ammonia + oxidized ferredoxin | - |
? | |
| nitrite + reduced ferredoxin | - |
[Chlorella] fusca | ammonia + oxidized ferredoxin | - |
? | |
| nitrite + reduced ferredoxin | - |
Cucurbita pepo | ammonia + oxidized ferredoxin | - |
? | |
| nitrite + reduced ferredoxin | - |
Anabaena cylindrica | ammonia + oxidized ferredoxin | - |
? | |
| nitrite + reduced ferredoxin | - |
Dunaliella tertiolecta | ammonia + oxidized ferredoxin | - |
? | |
| nitrite + reduced ferredoxin | second enzyme of photosynthetic nitrate-reducing system | Synechococcus elongatus PCC 7942 = FACHB-805 | ammonia + oxidized ferredoxin | - |
? | |
| nitrite + reduced ferredoxin | second enzyme of photosynthetic nitrate-reducing system | Spinacia oleracea | ammonia + oxidized ferredoxin | - |
? | |
| nitrite + reduced ferredoxin | second enzyme of photosynthetic nitrate-reducing system | [Chlorella] fusca | ammonia + oxidized ferredoxin | - |
? | |
| nitrite + reduced ferredoxin | second enzyme of photosynthetic nitrate-reducing system | Cucurbita pepo | ammonia + oxidized ferredoxin | - |
? | |
| nitrite + reduced ferredoxin | second enzyme of photosynthetic nitrate-reducing system | Anabaena cylindrica | ammonia + oxidized ferredoxin | - |
? | |
| nitrite + reduced ferredoxin | second enzyme of photosynthetic nitrate-reducing system | Dunaliella tertiolecta | ammonia + oxidized ferredoxin | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
stable | Spinacia oleracea |
| 60 | - |
10 min, more than 95% loss of activity | Spinacia oleracea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
Spinacia oleracea |
| 7 | 8 | - |
[Chlorella] fusca |
| 7 | 8 | - |
Cucurbita pepo |
| 7 | 8 | - |
Anabaena cylindrica |
| 7 | 8 | - |
Dunaliella tertiolecta |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| siroheme | prosthetic group | Spinacia oleracea | |
| siroheme | 1 mol siroheme per mol of enzyme | Spinacia oleracea | |
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Release 2023.1 (January 2023)
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