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Literature summary for 1.7.6.1 extracted from

  • Knipp, M.; Zhang, H.; Berry, R.E.; Walker, F.A.
    Overexpression in Escherichia coli and functional reconstitution of the liposome binding ferriheme protein nitrophorin 7 from the bloodsucking bug Rhodnius prolixus (2007), Protein Expr. Purif., 54, 183-191.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.7.6.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21. Expression and reconstitution method for NP7 that yields sufficient amounts of pure protein for extensive characterization Rhodnius prolixus

General Stability

General Stability Organism
NP7 is unstable when lyophilized Rhodnius prolixus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20966
-
 x * 20966, MALDI-TOF-MS Rhodnius prolixus
20969
-
 x * 20969, calculated from sequence Rhodnius prolixus

Organism

Organism UniProt Comment Textmining
Rhodnius prolixus Q6PQK2
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rhodnius prolixus

Source Tissue

Source Tissue Comment Organism Textmining
saliva
-
 Rhodnius prolixus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information NP7 is unique in its ability to bind to negatively charged cell surfaces Rhodnius prolixus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 20966, MALDI-TOF-MS Rhodnius prolixus
? x * 20969, calculated from sequence Rhodnius prolixus

Synonyms

Synonyms Comment Organism
Nitrophorin 7
-
 Rhodnius prolixus
NP7
-
 Rhodnius prolixus

Cofactor

Cofactor Comment Organism Structure
heme b nitrophorins bind the heme b cofactor in the beta-barrel of their lipocalin fold, which is further anchored through a proximal histidine-Fe(III) bond. Ferriheme is not reduced by excess NO Rhodnius prolixus