Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Rhodnius prolixus |
Protein Variants | Comment | Organism |
---|---|---|
K149A | using 3:1 phosphatidylcholine:phosphatidylserine vesicles, binding is reduced by a factor of three from that observed with wild-type protein, suggesting that this region does play an important role in phospholipid binding | Rhodnius prolixus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20600 | - |
x * 20600, calculated from sequence | Rhodnius prolixus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodnius prolixus | Q6PQK2 | - |
- |
Purification (Comment) | Organism |
---|---|
the NP7 protein is obtained as inclusion bodies and is denatured, refolded, and reconstituted with heme as described for other nitrophorins. The refolded reconstituted protein is purified by a two-step procedure | Rhodnius prolixus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
saliva | the salivary protein binds with high affinity to anionic phospholipid membranes. The protein is apparently targeted to the negatively charged surfaces of activated platelets and other cells where it can serve as a vasodilator, antihistamine, platelet aggregation inhibitor, and anticoagulant | Rhodnius prolixus | - |
Subunits | Comment | Organism |
---|---|---|
? | x * 20600, calculated from sequence | Rhodnius prolixus |
Synonyms | Comment | Organism |
---|---|---|
Nitrophorin 7 | - |
Rhodnius prolixus |
NP7 | - |
Rhodnius prolixus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 5 | the enzyme-NO complex is more stable at pH 4-5 than at physiologic pH. pH. In pH 4.5 sodium acetate buffer, a complex formed by incubation with S-nitroso-N-acetylpenicillamine is stable for several hours at room temperature, is purified by gel filtration chromatography at pH 4.5, and maintains indefinitely at -20°C. Raising the pH of the complex solution by adding concentrated Tris-HCl, pH 7.5 results in dissociation of the complex over a period of 10-15 min | Rhodnius prolixus |
7.5 | - |
the enzyme-NO complex is more stable at pH 4-5 than at physiologic pH. pH. In pH 4.5 sodium acetate buffer, a complex formed by incubation with S-nitroso-N-acetylpenicillamine is stable for several hours at room temperature, is purified by gel filtration chromatography at pH 4.5, and maintains indefinitely at -20°C. Raising the pH of the complex solution by adding concentrated Tris-HCl, pH 7.5 results in dissociation of the complex over a period of 10-15 min | Rhodnius prolixus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rhodnius prolixus | calculated from sequence | - |
8.72 |
General Information | Comment | Organism |
---|---|---|
physiological function | NP7 inhibits prothrombin activation by blocking phospholipid binding sites for the prothrombinase complex on the surfaces of vesicles and activated platelets. As a NO complex, NP7 inhibits collagen and ADP-induced platelet aggregation and induces disaggregation of ADP-stimulated platelets by an NO-mediated mechanism | Rhodnius prolixus |