Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.7.5.2 extracted from

  • Suharti; Heering, H.A.; de Vries, S.
    NO reductase from Bacillus azotoformans is a bifunctional enzyme accepting electrons from menaquinol and a specific endogenous membrane-bound cytochrome c551 (2004), Biochemistry, 43, 13487-13495.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
cytochrome c551 noncompetitive inhibitor of nitric oxide reduction when menaquinol is used as an electron donor Schinkia azotoformans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0045
-
cytochrome c551 pH and temperature not specified in the publication Schinkia azotoformans
0.141
-
juglone pH and temperature not specified in the publication Schinkia azotoformans
0.256
-
Plumbagin pH and temperature not specified in the publication Schinkia azotoformans

Metals/Ions

Metals/Ions Comment Organism Structure
copper the enzyme contains a CuA center in its small subunit Schinkia azotoformans

Organism

Organism UniProt Comment Textmining
Schinkia azotoformans
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with lapachol, reduced horse heart cytochrome c, cytochrome c550, and cytochrome c552 Schinkia azotoformans ?
-
?
nitric oxide + cytochrome c551 the menaquinol pathway has a 4fold greater maximal activity than the pathway via cytochrome c551 Schinkia azotoformans ?
-
?
nitric oxide + juglone
-
Schinkia azotoformans ?
-
?
nitric oxide + menaquinol the menaquinol pathway has a 4fold greater maximal activity than the pathway via cytochrome c551 Schinkia azotoformans nitrous oxide + menaquinone + H2O
-
?
nitric oxide + plumbagin
-
Schinkia azotoformans ?
-
?

Synonyms

Synonyms Comment Organism
menaquinol-dependent nitric oxide reductase
-
Schinkia azotoformans
NO reductase
-
Schinkia azotoformans
qCuANOR
-
Schinkia azotoformans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
41
-
nitric oxide using cytochrome c551 as electron donor, pH and temperature not specified in the publication Schinkia azotoformans
118
-
nitric oxide using juglone as electron donor, pH and temperature not specified in the publication Schinkia azotoformans
150
-
nitric oxide using plumbagin as electron donor, pH and temperature not specified in the publication Schinkia azotoformans
153
-
nitric oxide using menaquinol as electron donor, pH and temperature not specified in the publication Schinkia azotoformans

General Information

General Information Comment Organism
physiological function the ability of qCuANOR to accept electrons from both menaquinol and cytochrome c551 may be related to the regulation of the rate of NO reduction especially as a defense mechanism of B. azotoformans against the toxicity of NO. The menaquinol pathway, which has a 4-fold greater maximal activity than the pathway via cytochrome c551, is used for NO detoxification, whereas electron donation via the endogenous cytochrome c involves the cytochrome b6f complex serving the bioenergetic needs of the organism Schinkia azotoformans