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Literature summary for 1.7.3.3 extracted from

  • Kotb, E.
    Characterization of a thermostable uricase isolated from Bacillus firmus DWD-33 and its application for uric acid quantification in human serum (2015), Protein Pept. Lett., 22, 402-409 .
    View publication on PubMed
Search for more literature for 1.7.3.3

Application

Application Comment Organism
analysis uric acid can be detected in human sera with the enzyme with none of the tested uric acid analogs being a competitive substrate Cytobacillus firmus

General Stability

General Stability Organism
stable against oxidants and SDS Cytobacillus firmus

Inhibitors

Inhibitors Comment Organism Structure
2,9-Dimethyl-1,10-phenanthroline 0.1 mM, 64% residual activity Cytobacillus firmus
Cu2+ 1 mM, 15% residual activity Cytobacillus firmus
EDTA 20 mM, 1% residual activity Cytobacillus firmus
Fe3+ 1 mM, 36% residual activity Cytobacillus firmus
H2O2 purified uricase retains 72% of its original activity after incubation with 0.5% H2O2 for 6 h Cytobacillus firmus
Hg2+ 1 mM, 2% residual activity Cytobacillus firmus
additional information not inhibitory: Tween-20 or Triton X-100 at 0.5% w/v Cytobacillus firmus
o-phenanthroline 2 mM, 4% residual activity Cytobacillus firmus
SDS purified uricase retains 82% of its original activity after incubation with 0.5% SDS for 6 h Cytobacillus firmus
Tween-80 0.5% w/v, 61% residual activity Cytobacillus firmus
Zn2+ 1 mM, 22% residual activity Cytobacillus firmus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.079
-
 Urate pH 8.0, 40°C Cytobacillus firmus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 1 mM, 138% of initial activity Cytobacillus firmus

Organism

Organism UniProt Comment Textmining
Cytobacillus firmus
-
-
-
Cytobacillus firmus DWD-33
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cytobacillus firmus

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
urate + O2 + H2O
-
 Cytobacillus firmus 5-hydroxyisourate + H2O2
-
 ?
urate + O2 + H2O
-
 Cytobacillus firmus DWD-33 5-hydroxyisourate + H2O2
-
 ?

Subunits

Subunits Comment Organism
? x * 33500, SDS-PAGE Cytobacillus firmus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Cytobacillus firmus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 stable up to Cytobacillus firmus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
21800
-
 Urate pH 8.0, 40°C Cytobacillus firmus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Cytobacillus firmus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 10
-
 Cytobacillus firmus

Expression

Organism Comment Expression
Cytobacillus firmus highest expression yields are obtained in medium containing 0.8% maltose and 1.2% soybean powder as carbon and nitrogen sources, respectively up

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
276000
-
 Urate pH 8.0, 40°C Cytobacillus firmus