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Literature summary for 1.7.3.3 extracted from

  • Alishah, K.; Asad, S.; Khajeh, K.; Akbari, N.
    Utilizing intein-mediated protein cleaving for purification of uricase, a multimeric enzyme (2016), Enzyme Microb. Technol., 93-94, 92-98 .
    View publication on PubMed
Search for more literature for 1.7.3.3

Application

Application Comment Organism
synthesis expression of the Escherichia-coli-codon-optimized gene as a fusion with the N-terminus of Mxe GyrA intein and chitin-binding domain for simple purification. After purification, the cleavage of the fusion protein is induced by adding DTT. Pure and properly folded uricase is obtained Aspergillus flavus

Cloned(Commentary)

Cloned (Comment) Organism
-
 Aspergillus flavus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
 H2O2 25°C, pH not specified in the publication Aspergillus flavus

Organism

Organism UniProt Comment Textmining
Aspergillus flavus Q00511
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
urate + O2 + H2O
-
 Aspergillus flavus 5-hydroxyisourate + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
Rasburicase
-
 Aspergillus flavus
Uaz
-
 Aspergillus flavus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 12 h, 50% residual activity, complete loss of activity after 54 h Aspergillus flavus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
9.3
-
 H2O2 25°C, pH not specified in the publication Aspergillus flavus

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus flavus calculated and isoelectric focusing
-
 6.8