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Literature summary for 1.7.3.3 extracted from

  • Xiong, R.; Umar, S.; Chen, J.
    Process for production of recombinant baboon uricase in Escherichia coli Rosetta (DE3) (2013), Biotechnol. Biotechnol. Equip., 27, 4141-4144 .
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Papio sp.

Protein Variants

Protein Variants Comment Organism
synthesis expression of baboon uricase gene attached with Trx and hexahistidine tags in Escherichia coli Rosetta (DE3). The final yield of mature baboon uricase is 136.0 mg/l with enzyme activity of 17.93 U/mg Papio sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0062
-
Urate pH 8.2, 37°C Papio sp.

Organism

Organism UniProt Comment Textmining
Papio sp.
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
urate + O2 + H2O
-
Papio sp. 5-hydroxyisourate + H2O2
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Papio sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
-
Papio sp.