Literature summary for 1.7.3.3 extracted from

Feng, J.; Wang, L.; Liu, H.; Yang, X.; Liu, L.; Xie, Y.; Liu, M.; Zhao, Y.; Li, X.; Wang, D.; Zhan, C.G.; Liao, F.
Crystal structure of Bacillus fastidious uricase reveals an unexpected folding of the C-terminus residues crucial for thermostability under physiological conditions (2015), Appl. Microbiol. Biotechnol., 99, 7973-7986 .

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Cloned(Commentary)

Cloned (Comment)	Organism
-	Metabacillus fastidiosus

Crystallization (Commentary)

Crystallization (Comment)	Organism
structure to 1.4 A resolution, showing a homotetramer containing two homodimers. In each homodimer H-bonds are found between residues E311 and Y249 and between Y319 and D257. Electrostatic interaction networks surround D307 plus R310 and intersubunit R3, K312 plus D257, E318 plus K242, and L322 plus R258	Metabacillus fastidiosus

Protein Variants

Protein Variants	Comment	Organism
D307R	mutant is an inactive homotetramer of lower solubility	Metabacillus fastidiosus
E318R	mutant is similar to S314Stop	Metabacillus fastidiosus
L322D	mutant behaves similar to wild-type	Metabacillus fastidiosus
L322R	mutant shows reduced activity and thermal stability at pH 7.4, enhanced stability at pH 9.2	Metabacillus fastidiosus
additional information	the folding of the C-terminal residues is crucial for thermal stability. When positive charge on residue R3 is eliminated, the mutants become inactive dimers	Metabacillus fastidiosus
R310E	displays the lowest stability at pH 7.4 or 9.2 among tested mutants and the strongest effects of pH values on thermal stability	Metabacillus fastidiosus
S314Stop	mutant is a homotetramer of about 25% activity, 5 % half-life at pH 7.4 but 100 % half-life at pH 9.2	Metabacillus fastidiosus
Y249F	thermal stability is reduced by 50fold at pH 7.4, by about 7fold at pH 9.2	Metabacillus fastidiosus
Y319F	thermal stability is reduced by one magnitude at pH 7.4, but by 2fold at pH 9.2	Metabacillus fastidiosus
Y319F/Y249F	mutant is an inactive homotetramer	Metabacillus fastidiosus

General Stability

General Stability	Organism
at 37°C, pH 7.4 half-life is about 3 weeks	Metabacillus fastidiosus
at 37°C, pH 9.2 half-life is about 6 weeks	Metabacillus fastidiosus

Organism

Organism	UniProt	Comment	Textmining
Metabacillus fastidiosus	C5HDG5	-	-
Metabacillus fastidiosus ATCC 29604	C5HDG5	-	-

Subunits

Subunits	Comment	Organism
tetramer	crystallization data	Metabacillus fastidiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
65	-	pH 9.2, half-life 8 min	Metabacillus fastidiosus

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Release 2023.1 (January 2023)