Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 95% inhibition at 1 mM | Microbacterium sp. | |
Hg2+ | 98% inhibition at 1 mM | Microbacterium sp. | |
Li+ | 18.5% inhibition at 1 mM | Microbacterium sp. | |
additional information | no inhibition by Cu2+, Fe3+, or Zn2+ | Microbacterium sp. | |
o-phenanthroline | 99% inhibition at 2 mM | Microbacterium sp. | |
SDS | 49% inhibition at 0.5% w/v | Microbacterium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.31 | - |
Urate | pH 8.5, 30°C | Microbacterium sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe3+ | activates | Microbacterium sp. | |
additional information | a metal ion is possibly strongly bound in the enzyme and forms part of the uricase structure | Microbacterium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
x * 34000, SDS-PAGE | Microbacterium sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
urate + O2 + H2O | Microbacterium sp. | - |
5-hydroxyisourate + H2O2 | - |
? | |
urate + O2 + H2O | Microbacterium sp. ZZJ4-1 | - |
5-hydroxyisourate + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Microbacterium sp. | - |
- |
- |
Microbacterium sp. ZZJ4-1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 19.7fold by ammonium sulfate fractionation, anion exchange exchange and hydrophobic interaction chromatography, and gel filtration | Microbacterium sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.32 | - |
purified enzyme | Microbacterium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
urate + O2 + H2O | - |
Microbacterium sp. | 5-hydroxyisourate + H2O2 | - |
? | |
urate + O2 + H2O | - |
Microbacterium sp. ZZJ4-1 | 5-hydroxyisourate + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 34000, SDS-PAGE | Microbacterium sp. |
Synonyms | Comment | Organism |
---|---|---|
urate oxidoreductase | - |
Microbacterium sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Microbacterium sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 55 | 20°C: about 80% of maximal activity, maximal activity at 30°C, 55°C: about 55% of maximal activity | Microbacterium sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the molecular structure of enzyme has a reversible change at a temperature between 30°C and 60°C | Microbacterium sp. |
65 | - |
30 min, the purified enzyme retains 98.9% of its original activity | Microbacterium sp. |
70 | - |
30 min, the purified enzyme retains 64% of its original activity | Microbacterium sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.01 | - |
Urate | pH 8.5, 30°C | Microbacterium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Microbacterium sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 11 | pH 6.5: about 40% of maximal activity, pH 10.0: about 50% of maximal activity | Microbacterium sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | - |
25°C, 18 h, about 60% loss of activity | Microbacterium sp. |
7 | 10 | purified enzyme, 25°C, 18 h, stable | Microbacterium sp. |
11 | - |
25°C, 18 h, about 60% loss of activity | Microbacterium sp. |