Cloned (Comment) | Organism |
---|---|
expression in Saccharomyces cerevisiae | Aspergillus flavus |
Crystallization (Comment) | Organism |
---|---|
recombinant enzyme in complex with inhibitor 8-azaxanthine in presence of O2 or Cl-, batch technique at room temperature, 10-15 mg/ml protein with an excess of 0.5-2 mg/ml of 8-azaxanthin in 50 mM Tris/HCl, pH 8.5, in the presence of 5-8% w/v PEG 8000 and 0.05 M NaCl, 24-48 h, X-ray diffraction structure determination and analysis at 1.6-1.7 A resolution | Aspergillus flavus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
8-Azaxanthine | competitive inhibitor, binding structure, overview | Aspergillus flavus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the catalytic mechanism of UOX does not imply any metal ion | Aspergillus flavus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
urate + O2 + H2O | Aspergillus flavus | - |
5-hydroxyisourate + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus flavus | Q00511 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
urate + O2 + H2O = 5-hydroxyisourate + H2O2 | catalytic mechanism, overview | Aspergillus flavus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
urate + O2 + H2O | - |
Aspergillus flavus | 5-hydroxyisourate + H2O2 | - |
? | |
urate + O2 + H2O | dioxygen-binding site structure, overview | Aspergillus flavus | 5-hydroxyisourate + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Uox | - |
Aspergillus flavus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the catalytic mechanism of UOX does not imply any cofactor | Aspergillus flavus |