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Literature summary for 1.7.3.3 extracted from
- Purification and properties of uricase from Candida sp. and its application in uric acid analysis in serum (1994), Appl. Biochem. Biotechnol., 47, 57-63.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | determining the urate concentration in blood and urine | Candida sp. (in: Saccharomycetales) |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | - |
Candida sp. (in: Saccharomycetales) |  |
| Cu2+ | - |
Candida sp. (in: Saccharomycetales) | |
| Fe3+ | - |
Candida sp. (in: Saccharomycetales) | |
| Hg2+ | - |
Candida sp. (in: Saccharomycetales) | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | 76000 | gel filtration | Candida sp. (in: Saccharomycetales) |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| urate + O2 + H2O | Candida sp. (in: Saccharomycetales) | - |
5-hydroxyisourate + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Candida sp. (in: Saccharomycetales) | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Candida sp. (in: Saccharomycetales) |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 12.2 | - |
- |
Candida sp. (in: Saccharomycetales) |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| urate + O2 + H2O | - |
Candida sp. (in: Saccharomycetales) | 5-hydroxyisourate + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 70000-76000, SDS-PAGE | Candida sp. (in: Saccharomycetales) |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Candida sp. (in: Saccharomycetales) |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
after 5 min 50% of initial activity | Candida sp. (in: Saccharomycetales) |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Candida sp. (in: Saccharomycetales) |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9.5 | - |
Candida sp. (in: Saccharomycetales) |
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