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Literature summary for 1.7.3.3 extracted from
- Purification and some properties of urate oxidase from nitrogen-fixing nodules of cowpea (1981), Biochim. Biophys. Acta, 659, 132-140.
General Stability
| General Stability | Organism |
|---|---|
| frozen enzyme retains complete activity | Vigna unguiculata |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,2'-dipyridyl | - |
Vigna unguiculata |  |
| adenine | - |
Vigna unguiculata | |
| Allantoic acid | weak | Vigna unguiculata | |
| allantoin | - |
Vigna unguiculata | |
| CN- | - |
Vigna unguiculata | |
| Co2+ | - |
Vigna unguiculata | |
| Cu2+ | - |
Vigna unguiculata | |
| EDTA | - |
Vigna unguiculata | |
| glutamine | - |
Vigna unguiculata | |
| glyoxylic acid | weak | Vigna unguiculata | |
| guanine | - |
Vigna unguiculata | |
| hypoxanthine | weak | Vigna unguiculata | |
| inosine 5'-monophosphate | - |
Vigna unguiculata | |
| Mg2+ | slight | Vigna unguiculata | |
| Mn2+ | - |
Vigna unguiculata | |
| NH4+ | - |
Vigna unguiculata | |
| o-phenanthroline | - |
Vigna unguiculata | |
| Salicylhydroxamic acid | - |
Vigna unguiculata | |
| Urea | weak | Vigna unguiculata | |
| xanthine | - |
Vigna unguiculata | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.018 | - |
uric acid | - |
Vigna unguiculata | |
| 0.029 | - |
O2 | - |
Vigna unguiculata | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | Fe3+: stimulation | Vigna unguiculata | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
gel filtration | Vigna unguiculata |
| 100000 | - |
gel filtration | Vigna unguiculata |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| urate + O2 + H2O | Vigna unguiculata | - |
5-hydroxyisourate + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vigna unguiculata | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Vigna unguiculata |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.6 | - |
- |
Vigna unguiculata |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 7 days, 40% loss of activity | Vigna unguiculata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| urate + O2 + H2O | - |
Vigna unguiculata | 5-hydroxyisourate + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | at pH 8.8 the enzyme associates to form a dimer of about 10000 | Vigna unguiculata |
| monomer | native enzyme at pH 7.5 is a monomer with MW 50000 | Vigna unguiculata |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 18 | 20 | 2 days, 70% loss of activity | Vigna unguiculata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | 10 | - |
Vigna unguiculata |
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