Any feedback?
Literature summary for 1.7.3.1 extracted from
- Crystal structure of hypothetical protein PA4202 from Pseudomonas aeruginosa PAO1 in complex with nitroethane as a nitroalkane substrate (2018), Biochem. Biophys. Res. Commun., 503, 330-337 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Pseudomonas aeruginosa |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| mutant enzyme H183S in complex with nitroethane and FMN, hanging drop vapor diffusion method, using 0.2 M ammonium fluoride, 20% (w/v) polyethylene glycol 3350 | Pseudomonas aeruginosa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H183C | the mutant shows reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
| H183S | the mutant shows reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.4 | - |
nitroethane | wild type enzyme, at 30°C, pH not specified in the publication | Pseudomonas aeruginosa |  |
| 5.8 | - |
1-Nitropropane | wild type enzyme, at 30°C, pH not specified in the publication | Pseudomonas aeruginosa | |
| 8.1 | - |
nitroethane | mutant enzyme H183S, at 30°C, pH not specified in the publication | Pseudomonas aeruginosa | |
| 8.4 | - |
nitroethane | mutant enzyme H183C, at 30°C, pH not specified in the publication | Pseudomonas aeruginosa | |
| 9.6 | - |
1-Nitropropane | mutant enzyme H183S, at 30°C, pH not specified in the publication | Pseudomonas aeruginosa | |
| 9.8 | - |
1-Nitropropane | mutant enzyme H183C, at 30°C, pH not specified in the publication | Pseudomonas aeruginosa | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-nitrobutane + H2O + O2 | - |
Pseudomonas aeruginosa | butyraldehyde + nitrite + H2O2 | - |
? | |
| 1-nitropropane + H2O + O2 | - |
Pseudomonas aeruginosa | propionaldehyde + nitrite + H2O2 | - |
? | |
| nitroethane + H2O | - |
Pseudomonas aeruginosa | ethanal + nitrite + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | x-ray crystallography | Pseudomonas aeruginosa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NAO | - |
Pseudomonas aeruginosa |
| PA4202 | - |
Pseudomonas aeruginosa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0006 | - |
1-Nitropropane | mutant enzyme H183C, at 30°C, pH not specified in the publication | Pseudomonas aeruginosa | |
| 0.00061 | - |
nitroethane | mutant enzyme H183C, at 30°C, pH not specified in the publication | Pseudomonas aeruginosa | |
| 0.00098 | - |
1-Nitropropane | mutant enzyme H183S, at 30°C, pH not specified in the publication | Pseudomonas aeruginosa | |
| 0.0011 | - |
nitroethane | mutant enzyme H183S, at 30°C, pH not specified in the publication | Pseudomonas aeruginosa | |
| 0.024 | - |
1-Nitropropane | wild type enzyme, at 30°C, pH not specified in the publication | Pseudomonas aeruginosa | |
| 0.031 | - |
nitroethane | wild type enzyme, at 30°C, pH not specified in the publication | Pseudomonas aeruginosa | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | - |
Pseudomonas aeruginosa | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000062 | - |
1-Nitropropane | mutant enzyme H183C, at 30°C, pH not specified in the publication | Pseudomonas aeruginosa | |
| 0.000073 | - |
nitroethane | mutant enzyme H183C, at 30°C, pH not specified in the publication | Pseudomonas aeruginosa | |
| 0.0001 | - |
1-Nitropropane | mutant enzyme H183S, at 30°C, pH not specified in the publication | Pseudomonas aeruginosa | |
| 0.00013 | - |
nitroethane | mutant enzyme H183S, at 30°C, pH not specified in the publication | Pseudomonas aeruginosa | |
| 0.0042 | - |
1-Nitropropane | wild type enzyme, at 30°C, pH not specified in the publication | Pseudomonas aeruginosa | |
| 0.0057 | - |
nitroethane | wild type enzyme, at 30°C, pH not specified in the publication | Pseudomonas aeruginosa | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
