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Literature summary for 1.7.3.1 extracted from

  • Fitzpatrick, P.F.
    Nitroalkane oxidase Structure and mechanism (2017), Arch. Biochem. Biophys., 632, 41-46 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.7.3.1

Protein Variants

Protein Variants Comment Organism
C397S the mutant shows reduced activity compared to the wild type enzyme Fusarium oxysporum
D402E the mutant shows severely reduced activity compared to the wild type enzyme Fusarium oxysporum
D402N the mutant shows reduced activity compared to the wild type enzyme Fusarium oxysporum
R409K the mutant shows severely reduced activity compared to the wild type enzyme Fusarium oxysporum
S171A the mutant shows reduced activity compared to the wild type enzyme Fusarium oxysporum
S276A the mutant shows severely reduced activity compared to the wild type enzyme Fusarium oxysporum
Y398F the mutant shows reduced activity compared to the wild type enzyme Fusarium oxysporum

Inhibitors

Inhibitors Comment Organism Structure
cyanide
-
 Fusarium oxysporum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1
-
 nitroethane mutant enzyme C397S, at pH 8.0 and 30°C Fusarium oxysporum
2.1
-
 nitroethane mutant enzyme S171A, at pH 8.0 and 30°C Fusarium oxysporum
2.3
-
 nitroethane wild type enzyme, at pH 8.0 and 30°C Fusarium oxysporum
3.3
-
 nitroethane mutant enzyme S276A, at pH 8.0 and 30°C Fusarium oxysporum
4.8
-
 nitroethane mutant enzyme Y398F, at pH 8.0 and 30°C Fusarium oxysporum
14.6
-
 nitroethane mutant enzyme D402E, at pH 8.0 and 30°C Fusarium oxysporum
26.3
-
 nitroethane mutant enzyme R409K, at pH 8.0 and 30°C Fusarium oxysporum

Organism

Organism UniProt Comment Textmining
Fusarium oxysporum Q8X1D8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-nitrohexane + H2O + O2
-
 Fusarium oxysporum ?
-
 ?
1-nitrooctane + H2O + O2
-
 Fusarium oxysporum ?
-
 ?
1-nitropropane + H2O + O2
-
 Fusarium oxysporum propionaldehyde + nitrite + H2O2
-
 ?
nitroethane + H2O + O2
-
 Fusarium oxysporum ethanal + nitrite + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
NAO
-
 Fusarium oxysporum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1
-
 nitroethane mutant enzyme S276A, at pH 8.0 and 30°C Fusarium oxysporum
2.6
-
 nitroethane mutant enzyme R409K, at pH 8.0 and 30°C Fusarium oxysporum
3.1
-
 nitroethane mutant enzyme D402E, at pH 8.0 and 30°C Fusarium oxysporum
5.4
-
 nitroethane mutant enzyme C397S, at pH 8.0 and 30°C Fusarium oxysporum
5.4
-
 nitroethane mutant enzyme S171A, at pH 8.0 and 30°C Fusarium oxysporum
11
-
 nitroethane mutant enzyme Y398F, at pH 8.0 and 30°C Fusarium oxysporum
15
-
 nitroethane wild type enzyme, at pH 8.0 and 30°C Fusarium oxysporum

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Fusarium oxysporum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.098
-
 nitroethane mutant enzyme R409K, at pH 8.0 and 30°C Fusarium oxysporum
0.21
-
 nitroethane mutant enzyme D402E, at pH 8.0 and 30°C Fusarium oxysporum
0.3
-
 nitroethane mutant enzyme S276A, at pH 8.0 and 30°C Fusarium oxysporum
2.2
-
 nitroethane mutant enzyme Y398F, at pH 8.0 and 30°C Fusarium oxysporum
2.6
-
 nitroethane mutant enzyme C397S, at pH 8.0 and 30°C Fusarium oxysporum
2.6
-
 nitroethane mutant enzyme S171A, at pH 8.0 and 30°C Fusarium oxysporum
6.3
-
 nitroethane wild type enzyme, at pH 8.0 and 30°C Fusarium oxysporum