Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fusarium oxysporum | Q8X1D8 | - |
- |
Fusarium oxysporum ATCC 695 | Q8X1D8 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Fusarium oxysporum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitroethane + H2O | solvent isotope and viscositiy effects: the kcat proton inventory is consistent with a single exchangeable proton in flight, while the kcat/KM is consistent with either a single proton in flight in the transition state or a medium effect. Increasing the solvent viscosity does not affect the kcat or kcat/KM value significantly, establishing that nitroethane binding is at equilibrium and that product release does not limit kcat | Fusarium oxysporum | ethanal + nitrite + H2O2 | - |
? | |
nitroethane + H2O | solvent isotope and viscositiy effects: the kcat proton inventory is consistent with a single exchangeable proton in flight, while the kcat/KM is consistent with either a single proton in flight in the transition state or a medium effect. Increasing the solvent viscosity does not affect the kcat or kcat/KM value significantly, establishing that nitroethane binding is at equilibrium and that product release does not limit kcat | Fusarium oxysporum ATCC 695 | ethanal + nitrite + H2O2 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.4 | - |
nitroethane | cosubstrate D2O, 30°C, pH 8.5 | Fusarium oxysporum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
nitroethane | cosubstrate D2O, 30°C, pH 8.5 | Fusarium oxysporum |