Any feedback?
Literature summary for 1.7.2.9 extracted from
- Multiheme hydroxylamine oxidoreductases produce NO during ammonia oxidation in methanotrophs (2020), Proc. Natl. Acad. Sci. USA, 117, 24459-24463 .
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0014 | - |
hydroxylamine | pH 7.5, 60°C | Methylacidiphilum fumariolicum |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 250000 | - |
and 500000, native PAGE | Methylacidiphilum fumariolicum |
| 500000 | - |
and 250000, native PAGE | Methylacidiphilum fumariolicum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hydroxylamine + 3 ferricytochrome c | Methylacidiphilum fumariolicum | - |
nitric oxide + 3 ferrocytochrome c + 3 H+ | - |
? | |
| hydroxylamine + 3 ferricytochrome c | Methylacidiphilum fumariolicum SolV | - |
nitric oxide + 3 ferrocytochrome c + 3 H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methylacidiphilum fumariolicum | - |
- |
- |
| Methylacidiphilum fumariolicum SolV | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hydroxylamine + 3 ferricytochrome c | - |
Methylacidiphilum fumariolicum | nitric oxide + 3 ferrocytochrome c + 3 H+ | - |
? | |
| hydroxylamine + 3 ferricytochrome c | - |
Methylacidiphilum fumariolicum SolV | nitric oxide + 3 ferrocytochrome c + 3 H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 83860, calculated from sequence | Methylacidiphilum fumariolicum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| haoA | - |
Methylacidiphilum fumariolicum |
| Mfumv2_2472 | - |
Methylacidiphilum fumariolicum |
| mHAO | - |
Methylacidiphilum fumariolicum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Methylacidiphilum fumariolicum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
active up to | Methylacidiphilum fumariolicum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.2 | - |
hydroxylamine | pH 7.5, 60°C | Methylacidiphilum fumariolicum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Methylacidiphilum fumariolicum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | 9 heme c moieties per subunit | Methylacidiphilum fumariolicum | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | in methanotrophs, mHAO efficiently removes hydroxylamine, which severely inhibits calcium-dependent and lanthanide-dependent methanol dehydrogenases | Methylacidiphilum fumariolicum |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5100 | - |
hydroxylamine | pH 7.5, 60°C | Methylacidiphilum fumariolicum | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
