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Literature summary for 1.7.2.8 extracted from

  • Maalcke, W.J.; Reimann, J.; de Vries, S.; Butt, J.N.; Dietl, A.; Kip, N.; Mersdorf, U.; Barends, T.R.; Jetten, M.S.; Keltjens, J.T.; Kartal, B.
    Characterization of anammox hydrazine dehydrogenase, a key N2-producing enzyme in the global nitrogen cycle (2016), J. Biol. Chem., 291, 17077-17092.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.7.2.8

Inhibitors

Inhibitors Comment Organism Structure
hydroxylamine strong competitive inhibitor Candidatus Kuenenia stuttgartiensis
nitric oxide strong competitive inhibitor Candidatus Kuenenia stuttgartiensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 hydrazine at pH 7.0 and 37°C Candidatus Kuenenia stuttgartiensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
 3 * 67000, SDS-PAGE Candidatus Kuenenia stuttgartiensis
200000
-
 gel filtration Candidatus Kuenenia stuttgartiensis
201800
-
 MALDI-TOF mass spectrometry Candidatus Kuenenia stuttgartiensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hydrazine + ferricytochrome c Candidatus Kuenenia stuttgartiensis highly specific for hydrazine oxidation activity N2 + ferrocytochrome c
-
 ?

Organism

Organism UniProt Comment Textmining
Candidatus Kuenenia stuttgartiensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q Sepharose column chromatography and hydroxyapatite column chromatography Candidatus Kuenenia stuttgartiensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.55
-
 crude extract, at pH 7.0 and 37°C Candidatus Kuenenia stuttgartiensis
8.4
-
 after 15fold purification, at pH 7.0 and 37°C Candidatus Kuenenia stuttgartiensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hydrazine + ferricytochrome c highly specific for hydrazine oxidation activity Candidatus Kuenenia stuttgartiensis N2 + ferrocytochrome c
-
 ?
additional information hydroxylamine does not serve as a substrate Candidatus Kuenenia stuttgartiensis ?
-
 ?

Subunits

Subunits Comment Organism
homotrimer 3 * 67000, SDS-PAGE Candidatus Kuenenia stuttgartiensis
homotrimer 3 * 62272, calculated from amino acid sequence Candidatus Kuenenia stuttgartiensis

Synonyms

Synonyms Comment Organism
HDH
-
 Candidatus Kuenenia stuttgartiensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3 6 hydrazine at pH 7.0 and 37°C Candidatus Kuenenia stuttgartiensis

Cofactor

Cofactor Comment Organism Structure
heme the enzyme possessesg eight bound heme molecules Candidatus Kuenenia stuttgartiensis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0025
-
 nitric oxide at pH 7.0 and 37°C Candidatus Kuenenia stuttgartiensis
0.0079
-
 hydroxylamine at pH 7.0 and 37°C Candidatus Kuenenia stuttgartiensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3500
-
 hydrazine at pH 7.0 and 37°C Candidatus Kuenenia stuttgartiensis