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Literature summary for 1.7.2.5 extracted from
- The energy-conserving nitric-oxide-reductase system in Paracoccus denitrificans. Distinction from the nitrite reductase that catalyses synthesis of nitric oxide and evidence from trapping experiments for nitric oxide as a free intermediate during denitrification (1989), Eur. J. Biochem., 179, 683-692.
General Stability
| General Stability | Organism |
|---|---|
| after one cycle of freezing to -20°C and thawing stable, after more cycles activity is lost | Paracoccus denitrificans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Antimycin | 100% inhibition at 0.005 mg per mg vesicle protein, no inhibition with isoascorbate plus 2,3,5,6-tetramethyl-1,4-benzendiamine as electron donors | Paracoccus denitrificans |  |
| Myxothiazol | 100% inhibition at 200 ng per mg vesicle protein, no inhibition with isoascorbate plus 2,3,5,6-tetramethyl-1,4-benzendiamine as electron donors | Paracoccus denitrificans | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Paracoccus denitrificans | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe | - |
Paracoccus denitrificans | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 nitric oxide + reduced acceptor | Paracoccus denitrificans | - |
nitrous oxide + acceptor + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paracoccus denitrificans | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.58 | - |
- |
Paracoccus denitrificans |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for 2 months | Paracoccus denitrificans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 nitric oxide + reduced acceptor | - |
Paracoccus denitrificans | nitrous oxide + acceptor + H2O | - |
? | |
| 2 nitric oxide + reduced acceptor | NADH, succinate or isoascorbate plus 2,3,5,6-tetramethyl-1,4-benzendiamine as electron donors | Paracoccus denitrificans | nitrous oxide + acceptor + H2O | - |
? | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | - |
Paracoccus denitrificans | |
| NADH | - |
Paracoccus denitrificans | |
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