Any feedback?
Literature summary for 1.7.2.5 extracted from
- Nitric oxide reductase. Purification from Paracoccus denitrificans with use of a single column and some characteristics [published erratum appears in J Biol Chem 1991 Aug 25;266(24):16256] (1991), J. Biol. Chem., 266, 10899-10905.
General Stability
| General Stability | Organism |
|---|---|
| 0.8% octyl glucoside is needed for solubilzation | Paracoccus denitrificans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NO | reversible inhibition | Paracoccus denitrificans |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe | - |
Paracoccus denitrificans | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 nitric oxide + reduced acceptor | Paracoccus denitrificans | - |
nitrous oxide + acceptor + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paracoccus denitrificans | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10 | - |
at pH 6.5 | Paracoccus denitrificans |
| 22 | - |
at pH 5.0 | Paracoccus denitrificans |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable | Paracoccus denitrificans |
| 4°C, 2 d, 50% activity, the inclusion of 0.01% 2-phenylethanol, 1 mM benzamidine and 100 nM pepstatin stabilizes | Paracoccus denitrificans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 nitric oxide + reduced acceptor | - |
Paracoccus denitrificans | nitrous oxide + acceptor + H2O | - |
? | |
| 2 nitric oxide + reduced acceptor | ascorbate/phenazine methosulfate, N,N,N',N'-tetramethyl-1,4-benzendiamine/ascorbate, 2,3,5,6-tetramethyl-1,4-benzendiamine/ascorbate and NADH as electron donors | Paracoccus denitrificans | nitrous oxide + acceptor + H2O | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Paracoccus denitrificans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | - |
Paracoccus denitrificans | |
| NADH | - |
Paracoccus denitrificans | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
