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Literature summary for 1.7.2.5 extracted from

  • Cramm, R.; Pohlmann, A.; Friedrich, B.
    Purification and characterization of the single-component nitric oxide reductase from Ralstonia eutropha H16 (1999), FEBS Lett., 460, 6-10.
    View publication on PubMed
Search for more literature for 1.7.2.5

Metals/Ions

Metals/Ions Comment Organism Structure
Fe
-
 Cupriavidus necator

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
 SDS-PAGE Cupriavidus necator
84500
-
 amino acid composition Cupriavidus necator

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 nitric oxide + reduced acceptor Cupriavidus necator
-
 nitrous oxide + acceptor + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Cupriavidus necator
-
 contains two NO reductase enzymes named norB and norZ, that are located the former on the megaplasmid pHG1 and the latter on chromosomal DNA
-
Cupriavidus necator
-
 formerly Alcaligenes eutrophus strain H16
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cupriavidus necator

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.16
-
 with NADH and 2,3-dimethoxy-5-methyl-1,4-benzoquinone as electron donors Cupriavidus necator
0.22
-
 with N,N,N',N'-tetramethyl-1,4-benzendiamine and ascorbate as electron donors Cupriavidus necator
0.78
-
 with NADH and duroquinone as electron donors Cupriavidus necator
1.53
-
 with NADH as electron donor Cupriavidus necator
2.27
-
 with NADH and menadione as electron donors Cupriavidus necator
2.79
-
 with ascorbate and phenazine methosulfate as electron donors Cupriavidus necator

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 nitric oxide + reduced acceptor
-
 Cupriavidus necator nitrous oxide + acceptor + H2O
-
 ?
2 nitric oxide + reduced acceptor ascorbate and phenazine methosulfate, NADH, NADH/menadione, NADH/duroquinone, NADH/2,3-dimethoxy-5-methyl-1,4-benzoquinone and N,N,N',N'-tetramethyl-1,4-benzendiamine/ascorbate as electron donors Cupriavidus necator nitrous oxide + acceptor + H2O
-
 ?

Subunits

Subunits Comment Organism
monomer no NorC gene upstream NorB, contains an extension including two additional transmembrane spans and a (putative) periplasmic domain Cupriavidus necator

Cofactor

Cofactor Comment Organism Structure
heme
-
 Cupriavidus necator
NADH
-
 Cupriavidus necator