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Literature summary for 1.7.2.5 extracted from
- Proton delivery in NO reduction by fungal nitric-oxide reductase. Cryogenic crystallography, spectroscopy, and kinetics of ferric-NO complexes of wild-type and mutant enzymes (2000), J. Biol. Chem., 275, 4816-4826.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| at cryogenic temperature -173°C wild-type, S286V- and S286T-mutant | Fusarium oxysporum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S286T | 7% activity of wild-type | Fusarium oxysporum |
| S286V | 1% activity of wild-type | Fusarium oxysporum |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe | - |
Fusarium oxysporum |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 nitric oxide + reduced acceptor | Fusarium oxysporum | - |
nitrous oxide + acceptor + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fusarium oxysporum | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 nitric oxide + reduced acceptor | - |
Fusarium oxysporum | nitrous oxide + acceptor + H2O | - |
? | |
| 2 nitric oxide + reduced acceptor | NAD(P)H and NAD(P)+ as electron donor/acceptor | Fusarium oxysporum | nitrous oxide + acceptor + H2O | - |
? | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | - |
Fusarium oxysporum | |
| NADH | - |
Fusarium oxysporum | |
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