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Literature summary for 1.7.2.2 extracted from

  • Bykov, D.; Plog, M.; Neese, F.
    Heme-bound nitroxyl, hydroxylamine, and ammonia ligands as intermediates in the reaction cycle of cytochrome c nitrite reductase a theoretical study (2014), J. Biol. Inorg. Chem., 19, 97-112 .
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Wolinella succinogenes Q9S1E5
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Wolinella succinogenes DSM 1740 Q9S1E5
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the first event in the second halfcycle is the reduction of the HNO intermediate, which is accomplished by two proton-coupled electron transfer reactions. Two isomeric radical intermediates, HNOH radial and H2NO radical, are formed. Both intermediates are readily transformed into hydroxylamine, most likely through intramolecular proton transfer from either Arg114 or His277. An extra proton must enter the active site of the enzyme to initiate heterolytic cleavage of the N-O bond. As a result of N-O bond cleavage, the H2N+ intermediate is formed. It picks up an electron, forming H2N+ radical, which in turn reacts with Tyr218. The intramolecular reaction with Tyr218 in the final step of the nitrite reduction process leads directly to the final product, ammonia. Dissociation of the final product proceeds concomitantly with a change in spin state Wolinella succinogenes ?
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additional information the first event in the second halfcycle is the reduction of the HNO intermediate, which is accomplished by two proton-coupled electron transfer reactions. Two isomeric radical intermediates, HNOH radial and H2NO radical, are formed. Both intermediates are readily transformed into hydroxylamine, most likely through intramolecular proton transfer from either Arg114 or His277. An extra proton must enter the active site of the enzyme to initiate heterolytic cleavage of the N-O bond. As a result of N-O bond cleavage, the H2N+ intermediate is formed. It picks up an electron, forming H2N+ radical, which in turn reacts with Tyr218. The intramolecular reaction with Tyr218 in the final step of the nitrite reduction process leads directly to the final product, ammonia. Dissociation of the final product proceeds concomitantly with a change in spin state Wolinella succinogenes DSM 1740 ?
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?