Literature summary for 1.7.2.2 extracted from

Lockwood, C.W.; Burlat, B.; Cheesman, M.R.; Kern, M.; Simon, J.; Clarke, T.A.; Richardson, D.J.; Butt, J.N.
Resolution of key roles for the distal pocket histidine in cytochrome C nitrite reductases (2015), J. Am. Chem. Soc., 137, 3059-3068 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Escherichia coli
expression in Escherichia coli	Wolinella succinogenes

Crystallization (Commentary)

Crystallization (Comment)	Organism
mutant H264N, to 2.15 A resolution. Homodimeric protein, the constellation of His/His and Lys-coordinated c-hemes is indistinguishable from those of the native enzyme	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
H264N	mutant is unable to catalyze nitrite reduction but able to reduce hydroxylamine. The mutant simultaneously binds nitrite and electrons at the catalytic heme	Escherichia coli
H277N	mutant is unable to catalyze nitrite reduction but able to reduce hydroxylamine. The mutant simultaneously binds nitrite and electrons at the catalytic heme	Wolinella succinogenes

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.046	-	nitrite	wild-type, pH 7.0, temperature not specified in the publication	Escherichia coli
0.08	-	nitrite	wild-type, pH 7.0, temperature not specified in the publication	Wolinella succinogenes
2.01	-	hydroxylamine	wild-type, pH 7.0, temperature not specified in the publication	Wolinella succinogenes
2.03	-	hydroxylamine	mutant H264N, pH 7.0, temperature not specified in the publication	Wolinella succinogenes
30	-	hydroxylamine	wild-type, pH 7.0, temperature not specified in the publication	Escherichia coli
170	-	hydroxylamine	mutant H264N, pH 7.0, temperature not specified in the publication	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P0ABK9	-	-
Wolinella succinogenes	Q9S1E5	-	-
Wolinella succinogenes DSM 1740	Q9S1E5	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
hydroxylamine + reduced benzyl viologen	-	Escherichia coli	? + oxidized benzyl viologen	-	?
hydroxylamine + reduced benzyl viologen	-	Wolinella succinogenes	? + oxidized benzyl viologen	-	?
hydroxylamine + reduced benzyl viologen	-	Wolinella succinogenes DSM 1740	? + oxidized benzyl viologen	-	?
nitrite + reduced methyl viologen	-	Escherichia coli	NH3 + H2O + oxidized methyl viologen	-	?
nitrite + reduced methyl viologen	-	Wolinella succinogenes	NH3 + H2O + oxidized methyl viologen	-	?
nitrite + reduced methyl viologen	-	Wolinella succinogenes DSM 1740	NH3 + H2O + oxidized methyl viologen	-	?

Synonyms

Synonyms	Comment	Organism
cytochrome c-552	-	Escherichia coli
cytochrome c-552	-	Wolinella succinogenes
NrfA	-	Escherichia coli
NrfA	-	Wolinella succinogenes

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
197	-	hydroxylamine	mutant H264N, pH 7.0, temperature not specified in the publication	Wolinella succinogenes
215	-	hydroxylamine	wild-type, pH 7.0, temperature not specified in the publication	Wolinella succinogenes
758	-	nitrite	wild-type, pH 7.0, temperature not specified in the publication	Wolinella succinogenes
1350	-	nitrite	wild-type, pH 7.0, temperature not specified in the publication	Escherichia coli
3100	-	hydroxylamine	mutant H264N, pH 7.0, temperature not specified in the publication	Escherichia coli
3160	-	hydroxylamine	wild-type, pH 7.0, temperature not specified in the publication	Escherichia coli

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Release 2023.1 (January 2023)