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Literature summary for 1.7.2.2 extracted from
- Molecular and catalytic properties of a novel cytochrome c nitrite reductase from nitrate-reducing haloalkaliphilic sulfur-oxidizing bacterium Thioalkalivibrio nitratireducens (2006), Biochim. Biophys. Acta, 1764, 715-723.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| azide | - |
Thioalkalivibrio nitratireducens |  |
| cyanide | strong inhibitor | Thioalkalivibrio nitratireducens | |
| nitrate | - |
Thioalkalivibrio nitratireducens | |
| phosphate | weak inhibitor | Thioalkalivibrio nitratireducens | |
| sulfate | weak inhibitor | Thioalkalivibrio nitratireducens | |
| sulfite | - |
Thioalkalivibrio nitratireducens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 16.4 | - |
hydroxylamine | in 0.1 M K+-phosphate buffer, at pH 7.0 and 30°C | Thioalkalivibrio nitratireducens | |
| 16.7 | - |
nitrite | in 0.1 M K+-phosphate buffer, at pH 7.0 and 30°C | Thioalkalivibrio nitratireducens | |
| 60 | - |
sulfite | in 0.1 M K+-phosphate buffer, at pH 7.0 and 30°C | Thioalkalivibrio nitratireducens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1-3 mol per subunit | Thioalkalivibrio nitratireducens | |
| Fe | 8-11 mol per subunit | Thioalkalivibrio nitratireducens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64000 | - |
6 * 64000, calculated from sequence analysis and spectral properties | Thioalkalivibrio nitratireducens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thioalkalivibrio nitratireducens | - |
- |
- |
| Thioalkalivibrio nitratireducens ALEN 2 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| non-denaturing preparative polyacrylamide gel electrophoresis | Thioalkalivibrio nitratireducens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4080 | - |
- |
Thioalkalivibrio nitratireducens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 30°C, potassium phosphate buffer at pH 7.0, 24 h, no loss of activity | Thioalkalivibrio nitratireducens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hydroxylamine + ferrocytochrome c | 10 mM hydroxylamine | Thioalkalivibrio nitratireducens | NH3 + ferricytochrome c + H2O | - |
ir | |
| hydroxylamine + ferrocytochrome c | 10 mM hydroxylamine | Thioalkalivibrio nitratireducens ALEN 2 | NH3 + ferricytochrome c + H2O | - |
ir | |
| additional information | no reaction with chloride, bromate and nitrate | Thioalkalivibrio nitratireducens | ? | - |
? | |
| additional information | the enzyme possesses also peroxidase activity | Thioalkalivibrio nitratireducens | ? | - |
? | |
| additional information | no reaction with chloride, bromate and nitrate | Thioalkalivibrio nitratireducens ALEN 2 | ? | - |
? | |
| additional information | the enzyme possesses also peroxidase activity | Thioalkalivibrio nitratireducens ALEN 2 | ? | - |
? | |
| nitrite + ferrocytochrome c | - |
Thioalkalivibrio nitratireducens | NH3 + ferricytochrome c + H2O | - |
? | |
| nitrite + ferrocytochrome c | - |
Thioalkalivibrio nitratireducens ALEN 2 | NH3 + ferricytochrome c + H2O | - |
? | |
| sulfite + ? | - |
Thioalkalivibrio nitratireducens | sulfide + ? | - |
? | |
| sulfite + ? | - |
Thioalkalivibrio nitratireducens ALEN 2 | sulfide + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 64000, calculated from sequence analysis and spectral properties | Thioalkalivibrio nitratireducens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ccNiR | - |
Thioalkalivibrio nitratireducens |
| cytochrome c nitrite reductase | - |
Thioalkalivibrio nitratireducens |
| multihaem c NiR | - |
Thioalkalivibrio nitratireducens |
| TvNiR | - |
Thioalkalivibrio nitratireducens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Thioalkalivibrio nitratireducens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
at an enzyme concentration of 2 mg/ml in potassium phosphate buffer pH 7.0 the enzyme is stable at 30°C for at least 24 h | Thioalkalivibrio nitratireducens |
| 50 | - |
incubation at 50°C results in 1.5fold increase of activity | Thioalkalivibrio nitratireducens |
| 70 | - |
at 70°C the enzyme looses 50% of its activity in about 3 h | Thioalkalivibrio nitratireducens |
| 80 | - |
at 80°C activity decreases by 50% in 10min | Thioalkalivibrio nitratireducens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 10 | - |
Thioalkalivibrio nitratireducens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | 7.5 units heme per subunit | Thioalkalivibrio nitratireducens | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
cyanide | - |
Thioalkalivibrio nitratireducens | |
| 60 | - |
sulfite | - |
Thioalkalivibrio nitratireducens | |
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