Literature summary for 1.7.2.1 extracted from

Hira, D.; Toh, H.; Migita, C.T.; Okubo, H.; Nishiyama, T.; Hattori, M.; Furukawa, K.; Fujii, T.
Anammox organism KSU-1 expresses a NirK-type copper-containing nitrite reductase instead of a NirS-type with cytochrome cd1 (2012), FEBS Lett., 586, 1658-1663.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	uncultured bacterium

Crystallization (Commentary)

Crystallization (Comment)	Organism
homology modeling based on PDB entry 1BKW. Model demonstrates that the enzyme can be folded into two cupredoxin domains of the well-known CuNIR structure and implies that the conserved residues H122, D125, H127, H158, C159, H168, M173,H272, and H321 form the type 1 and type 2 Cu sites	uncultured bacterium

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.25	-	nitrite	pH 6.5, 25°C	uncultured bacterium

Metals/Ions

Metals/Ions	Comment	Organism	Structure
copper	copper atoms are coordinated by H122, C159, H168, and M173 for the type 1 Cu site and H127, H158, and H321 for the type 2 Cu site. The absorption spectrum of NirK displays two peaks at 598 and 449 nm characteristic for type I Cu proteins	uncultured bacterium

Organism

Organism	UniProt	Comment	Textmining
uncultured bacterium	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
nitrite + reduced benzyl viologen	-	uncultured bacterium	nitric oxide + oxidized benzyl viologen	-	?

Synonyms

Synonyms	Comment	Organism
NirK	-	uncultured bacterium

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Release 2023.1 (January 2023)