Cloned (Comment) | Organism |
---|---|
- |
Hyphomicrobium denitrificans |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion, 0.002 ml protein solution containing 20 mg/ml protein in 20 mM Tris-Hcl, pH 7.5 are mixed with reservoir solution containing 18% polyethylene glycol 4000 and 100 mM Tris-HCl, pH 8.9 at 20°C, crystals of wild-type HdNIR and C260A mutant diffract to 2.35 A and 3.5 A, respectively | Hyphomicrobium denitrificans |
Protein Variants | Comment | Organism |
---|---|---|
C114A | lacks the type I copper ion in the N-terminal domain, shows catalytic activity | Hyphomicrobium denitrificans |
C260A | lacks the type I copper ion in the C-terminal domain, no nitrite-reduction activity | Hyphomicrobium denitrificans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | enzyme contains 2 type I copper and 1 type II copper ions | Hyphomicrobium denitrificans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
6 * 50000 | Hyphomicrobium denitrificans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrite + reduced electron donor | Hyphomicrobium denitrificans | - |
NO + H2O + oxidized electron donor | - |
? | |
nitrite + reduced electron donor | Hyphomicrobium denitrificans A3151 | - |
NO + H2O + oxidized electron donor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hyphomicrobium denitrificans | - |
A3151 | - |
Hyphomicrobium denitrificans A3151 | - |
A3151 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Hyphomicrobium denitrificans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrite + reduced azurin | - |
Hyphomicrobium denitrificans | NO + H2O + oxidized azurin | - |
? | |
nitrite + reduced azurin | - |
Hyphomicrobium denitrificans A3151 | NO + H2O + oxidized azurin | - |
? | |
nitrite + reduced electron donor | - |
Hyphomicrobium denitrificans | NO + H2O + oxidized electron donor | - |
? | |
nitrite + reduced electron donor | - |
Hyphomicrobium denitrificans A3151 | NO + H2O + oxidized electron donor | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 50000 | Hyphomicrobium denitrificans |
Synonyms | Comment | Organism |
---|---|---|
copper-containing nitrite reductase | - |
Hyphomicrobium denitrificans |
HdNIR | - |
Hyphomicrobium denitrificans |