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Literature summary for 1.7.2.1 extracted from

  • Xie, Y.; Inoue, T.; Seike, N.; Matsumura, H.; Kanbayashi, K.; Itoh, K.; Kataoka, K.; Yamaguchi, K.; Suzuki, S.; Kai, Y.; Deligeer
    Crystallization and preliminary x-ray crystallographic studies of dissimilatory nitrite reductase isolated from Hyphomicrobium denitrificans A3151 (2004), Acta Crystallogr. Sect. D, D60, 2383-2386.
    View publication on PubMed
Search for more literature for 1.7.2.1

Cloned(Commentary)

Cloned (Comment) Organism
-
 Hyphomicrobium denitrificans

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion, 0.002 ml protein solution containing 20 mg/ml protein in 20 mM Tris-Hcl, pH 7.5 are mixed with reservoir solution containing 18% polyethylene glycol 4000 and 100 mM Tris-HCl, pH 8.9 at 20°C, crystals of wild-type HdNIR and C260A mutant diffract to 2.35 A and 3.5 A, respectively Hyphomicrobium denitrificans

Protein Variants

Protein Variants Comment Organism
C114A lacks the type I copper ion in the N-terminal domain, shows catalytic activity Hyphomicrobium denitrificans
C260A lacks the type I copper ion in the C-terminal domain, no nitrite-reduction activity Hyphomicrobium denitrificans

Metals/Ions

Metals/Ions Comment Organism Structure
copper enzyme contains 2 type I copper and 1 type II copper ions Hyphomicrobium denitrificans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 6 * 50000 Hyphomicrobium denitrificans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nitrite + reduced electron donor Hyphomicrobium denitrificans
-
 NO + H2O + oxidized electron donor
-
 ?
nitrite + reduced electron donor Hyphomicrobium denitrificans A3151
-
 NO + H2O + oxidized electron donor
-
 ?

Organism

Organism UniProt Comment Textmining
Hyphomicrobium denitrificans
-
 A3151
-
Hyphomicrobium denitrificans A3151
-
 A3151
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Hyphomicrobium denitrificans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitrite + reduced azurin
-
 Hyphomicrobium denitrificans NO + H2O + oxidized azurin
-
 ?
nitrite + reduced azurin
-
 Hyphomicrobium denitrificans A3151 NO + H2O + oxidized azurin
-
 ?
nitrite + reduced electron donor
-
 Hyphomicrobium denitrificans NO + H2O + oxidized electron donor
-
 ?
nitrite + reduced electron donor
-
 Hyphomicrobium denitrificans A3151 NO + H2O + oxidized electron donor
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 50000 Hyphomicrobium denitrificans

Synonyms

Synonyms Comment Organism
copper-containing nitrite reductase
-
 Hyphomicrobium denitrificans
HdNIR
-
 Hyphomicrobium denitrificans