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Literature summary for 1.7.2.1 extracted from

  • Ichiki, H.; Tanaka, Y.; Mochizuki, K.; Yoshimatsu, K.; Sakurai, T.; Fujiwara, T.
    Purification, characterization, and genetic analysis of Cu-containing dissimilatory nitrite reductase from a denitrifying halophilic archaeon, Haloarcula marismortui (2001), J. Bacteriol., 183, 4149-4156.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
diethyldithiocarbamate 1 mM, complete inhibition Haloarcula marismortui
EDTA 1 mM, 70% inhibition after 1 h Haloarcula marismortui
KCN 1 mM, complete inhibition Haloarcula marismortui

Metals/Ions

Metals/Ions Comment Organism Structure
copper blue copper enzyme, 1 type 1 and 1 type 2 Cu atoms per subunit Haloarcula marismortui
copper green copper enzyme Haloferax denitrificans
copper green copper enzyme Achromobacter cycloclastes
copper blue copper enzyme Fusarium oxysporum
copper blue copper enzyme Achromobacter xylosoxidans
KCl 2 M, enzyme is activated by high salt concentrations Haloarcula marismortui
LiCl 2 M, enzyme is activated by high salt concentrations Haloarcula marismortui
NaNO3 2 M, enzyme is activated by high salt concentrations Haloarcula marismortui
NH4Cl 2 M, enzyme is activated by high salt concentrations Haloarcula marismortui

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36500
-
3 * 36500, calculated from sequence, crystal structure Achromobacter xylosoxidans
37000
-
3 * 37000, calculated from sequence, crystal structure Achromobacter cycloclastes
41800
-
2 * 41800, SDS-PAGE Fusarium oxysporum
46000
-
3 * 46000, SDS-PAGE Haloarcula marismortui
64000
-
2 * 64000, SDS-PAGE Haloferax denitrificans
69000
-
gel filtration Achromobacter cycloclastes
70000
-
gel filtration Achromobacter xylosoxidans
83300
-
gel filtration Fusarium oxysporum
103000
-
sedimentation equilibrium Achromobacter xylosoxidans
105000
-
sedimentation equlibrium Achromobacter cycloclastes
127000
-
gel filtration Haloferax denitrificans
167000
-
gel filtration Haloarcula marismortui

Organism

Organism UniProt Comment Textmining
Achromobacter cycloclastes P25006
-
-
Achromobacter xylosoxidans
-
-
-
Fusarium oxysporum
-
-
-
Haloarcula marismortui
-
-
-
Haloferax denitrificans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
butyl-toyopearl, Sepharose CL-6B, Sephacryl S-300, octyl-sepharose, hydroxyapatite Haloarcula marismortui

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
960
-
in the presence of 2 M NaCl Haloarcula marismortui

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitrite + ferrocytochrome c
-
Haloarcula marismortui nitric oxide + H2O + ferricytochrome c
-
?
nitrite + ferrocytochrome c
-
Fusarium oxysporum nitric oxide + H2O + ferricytochrome c
-
?
nitrite + ferrocytochrome c
-
Achromobacter xylosoxidans nitric oxide + H2O + ferricytochrome c
-
?
nitrite + ferrocytochrome c
-
Haloferax denitrificans nitric oxide + H2O + ferricytochrome c
-
?
nitrite + ferrocytochrome c
-
Achromobacter cycloclastes nitric oxide + H2O + ferricytochrome c
-
?
NO2- + Na2S2O4 pysiological electron donor is unknown, no activity with methyl viologen, phenazine methosulfate or N,N,N',N',-tetramethyl-p-phenylenediamine Haloarcula marismortui NO + Na2S2O3
-
?

Subunits

Subunits Comment Organism
dimer 2 * 64000, SDS-PAGE Haloferax denitrificans
dimer 2 * 41800, SDS-PAGE Fusarium oxysporum
trimer 3 * 46000, SDS-PAGE Haloarcula marismortui
trimer 3 * 37000, calculated from sequence, crystal structure Achromobacter cycloclastes
trimer 3 * 36500, calculated from sequence, crystal structure Achromobacter xylosoxidans