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Literature summary for 1.7.2.1 extracted from

  • Richter, C.D.; Allen, J.W.A.; Higham, C.W.; Koppenhofer, A.; Zajicek, R.S.; Watmough, N.J.; Ferguson, S.J.
    Cytochrome cd1, reductive activation and kinetic analysis of a multifunctional respiratory enzyme (2002), J. Biol. Chem., 277, 3093-3100.
    View publication on PubMed
Search for more literature for 1.7.2.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
 nitrite 25°C, pH 7.0, electron donor cytochrome c550 Paracoccus pantotrophus
0.007
-
 nitrite 25°C, pH 7.0, electron donor horse heart cytochrome c Paracoccus pantotrophus
0.0073
-
 NO2- initially oxidized enzyme, electron donor horse heart cytochrome c Paracoccus pantotrophus
0.011
-
 NO2- pre-reduced enzyme, electron donor horse heart cytochrome c Paracoccus pantotrophus
0.011
-
 nitrite 25°C, pH 7.0, pre-reduced cytochrome cd1, electron donor horse heart cytochrome c Paracoccus pantotrophus
0.012
-
 NO2- pre-reduced enzyme, electron donor cytochrome c550 Paracoccus pantotrophus
0.012
-
 nitrite 25°C, pH 7.0, pre-reduced cytochrome cd1, electron donor cytochrome c550 Paracoccus pantotrophus
0.0187
-
 NO2- initially oxidized enzyme, electron donor pseudoazurin Paracoccus pantotrophus
0.019
-
 nitrite 25°C, pH 7.0, electron donor pseudoazurin Paracoccus pantotrophus
0.067
-
 NO2- initially oxidized enzyme, electron donor cytochrome c550 Paracoccus pantotrophus
0.071
-
 NO2- pre-reduced enzyme, electron donor pseudoazurin Paracoccus pantotrophus
0.071
-
 nitrite 25°C, pH 7.0, pre-reduced cytochrome cd1, electron donor pseudoazurin Paracoccus pantotrophus
0.148
-
 O2 pre-reduced enzyme, electron donor cytochrome c550 Paracoccus pantotrophus
0.15
-
 O2 25°C, pH 7.0, pre-reduced cytochrome cd1, electron donor cytochrome c550 Paracoccus pantotrophus
0.16
-
 O2 25°C, pH 7.0, pre-reduced cytochrome cd1, electron donor pseudoazurin Paracoccus pantotrophus
0.161
-
 O2 pre-reduced enzyme, electron donor pseudoazurin Paracoccus pantotrophus
0.166
-
 O2 pre-reduced enzyme, electron donor horse heart cytochrome c Paracoccus pantotrophus
0.17
-
 O2 25°C, pH 7.0, pre-reduced cytochrome cd1, electron donor horse heart cytochrome c Paracoccus pantotrophus
0.21
-
 O2 25°C, pH 7.0, electron donor cytochrome c550 Paracoccus pantotrophus
0.214
-
 O2 initially oxidized enzyme, electron donor cytochrome c550 Paracoccus pantotrophus
0.43
-
 hydroxylamine 25°C, pH 7.0, pre-reduced cytochrome cd1, electron donor horse heart cytochrome c Paracoccus pantotrophus
0.434
-
 NH2OH pre-reduced enzyme, electron donor horse heart cytochrome c Paracoccus pantotrophus
0.47
-
 O2 initially oxidized enzyme, electron donor horse heart cytochrome c Paracoccus pantotrophus
0.47
-
 O2 25°C, pH 7.0, electron donor horse heart cytochrome c Paracoccus pantotrophus
0.68
-
 NH2OH pre-reduced enzyme, electron donor cytochrome c550 Paracoccus pantotrophus
0.68
-
 hydroxylamine 25°C, pH 7.0, pre-reduced cytochrome cd1, electron donor cytochrome c550 Paracoccus pantotrophus
1.15
-
 NH2OH initially oxidized enzyme, electron donor horse heart cytochrome c Paracoccus pantotrophus
1.15
-
 hydroxylamine 25°C, pH 7.0, electron donor horse heart cytochrome c Paracoccus pantotrophus
1.38
-
 NH2OH initially oxidized enzyme, electron donor cytochrome c550 Paracoccus pantotrophus
1.38
-
 hydroxylamine 25°C, pH 7.0, electron donor cytochrome c550 Paracoccus pantotrophus
2.5
-
 NH2OH pre-reduced enzyme, electron donor pseudoazurin Paracoccus pantotrophus
2.5
-
 hydroxylamine 25°C, pH 7.0, pre-reduced cytochrome cd1, electron donor pseudoazurin Paracoccus pantotrophus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nitrite + reduced pseudoazurin Paracoccus pantotrophus
-
 NO + H2O + oxidized pseudoazurin
-
 ?

Organism

Organism UniProt Comment Textmining
Paracoccus pantotrophus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Paracoccus pantotrophus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hydroxylamine + reduced pseudoazurin
-
 Paracoccus pantotrophus NH3 + H2O + oxidized pseudoazurin
-
 ?
nitrite + reduced pseudoazurin
-
 Paracoccus pantotrophus NO + H2O + oxidized pseudoazurin
-
 ?
O2 + reduced pseudoazurin
-
 Paracoccus pantotrophus H2O + oxidized pseudoazurin
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.08
-
 NH2OH initially oxidized enzyme, electron donor cytochrome c550 Paracoccus pantotrophus
0.08
-
 hydroxylamine 25°C, pH 7.0, electron donor cytochrome c550 Paracoccus pantotrophus
0.11
-
 O2 initially oxidized enzyme, electron donor horse heart cytochrome c Paracoccus pantotrophus
0.11
-
 O2 25°C, pH 7.0, electron donor horse heart cytochrome c Paracoccus pantotrophus
0.17
-
 O2 initially oxidized enzyme, electron donor cytochrome c550 Paracoccus pantotrophus
0.17
-
 O2 25°C, pH 7.0, electron donor cytochrome c550 Paracoccus pantotrophus
0.2
-
 NH2OH initially oxidized enzyme, electron donor horse heart cytochrome c Paracoccus pantotrophus
0.2
-
 hydroxylamine 25°C, pH 7.0, electron donor horse heart cytochrome c Paracoccus pantotrophus
2.1
-
 NO2- initially oxidized enzyme, electron donor cytochrome c550 Paracoccus pantotrophus
2.1
-
 nitrite 25°C, pH 7.0, electron donor cytochrome c550 Paracoccus pantotrophus
2.4
-
 NO2- initially oxidized enzyme, electron donor horse heart cytochrome c Paracoccus pantotrophus
2.4
-
 nitrite 25°C, pH 7.0, electron donor horse heart cytochrome c Paracoccus pantotrophus
2.8
-
 O2 pre-reduced enzyme, electron donor horse heart cytochrome c Paracoccus pantotrophus
2.8
-
 O2 25°C, pH 7.0, pre-reduced cytochrome cd1, electron donor horse heart cytochrome c Paracoccus pantotrophus
3
-
 O2 pre-reduced enzyme, electron donor cytochrome c550 Paracoccus pantotrophus
3
-
 NH2OH pre-reduced enzyme, electron donor horse heart cytochrome c Paracoccus pantotrophus
3
-
 O2 25°C, pH 7.0, pre-reduced cytochrome cd1, electron donor cytochrome c550 Paracoccus pantotrophus
3
-
 hydroxylamine 25°C, pH 7.0, pre-reduced cytochrome cd1, electron donor horse heart cytochrome c Paracoccus pantotrophus
3.5
-
 NH2OH pre-reduced enzyme, electron donor cytochrome c550 Paracoccus pantotrophus
3.5
-
 hydroxylamine 25°C, pH 7.0, pre-reduced cytochrome cd1, electron donor cytochrome c550 Paracoccus pantotrophus
5.3
-
 NO2- initially oxidized enzyme, electron donor pseudoazurin Paracoccus pantotrophus
5.3
-
 nitrite 25°C, pH 7.0, electron donor pseudoazurin Paracoccus pantotrophus
6.4
-
 O2 pre-reduced enzyme, electron donor pseudoazurin Paracoccus pantotrophus
6.4
-
 NH2OH pre-reduced enzyme, electron donor pseudoazurin Paracoccus pantotrophus
6.4
-
 O2 25°C, pH 7.0, pre-reduced cytochrome cd1, electron donor pseudoazurin Paracoccus pantotrophus
6.4
-
 hydroxylamine 25°C, pH 7.0, pre-reduced cytochrome cd1, electron donor pseudoazurin Paracoccus pantotrophus
41
-
 NO2- pre-reduced enzyme, electron donor horse heart cytochrome c Paracoccus pantotrophus
41
-
 nitrite 25°C, pH 7.0, pre-reduced cytochrome cd1, electron donor horse heart cytochrome c Paracoccus pantotrophus
74
-
 NO2- pre-reduced enzyme, electron donor cytochrome c550 Paracoccus pantotrophus
74
-
 nitrite 25°C, pH 7.0, pre-reduced cytochrome cd1, electron donor cytochrome c550 Paracoccus pantotrophus
106
-
 NO2- all-ferric nitrite-bound complex, electron donor pseudoazurin Paracoccus pantotrophus
144
-
 NO2- pre-reduced enzyme, electron donor pseudoazurin Paracoccus pantotrophus
144
-
 nitrite 25°C, pH 7.0, pre-reduced cytochrome cd1, electron donor pseudoazurin Paracoccus pantotrophus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1 5.4 NH2OH reduction, electron donor pseudoazurin Paracoccus pantotrophus
5.1 5.4 hydroxylamine reduction, electron donor pseudoazurin, pre-reduced cytochrome cd1 Paracoccus pantotrophus
5.4
-
 NH2OH reduction, electron donor cytochrome c550 Paracoccus pantotrophus
5.4
-
 hydroxylamine reduction, electron donor cytochrome c550, pre-reduced cytochrome cd1 Paracoccus pantotrophus
5.7
-
 nitrite reduction, electron donor cytochrome c550 Paracoccus pantotrophus
5.7
-
 nitrite reduction, electron donor cytochrome c550, pre-reduced cytochrome cd1 Paracoccus pantotrophus
5.8
-
 nitrite reduction, electron donor pseudoazurin Paracoccus pantotrophus
5.8
-
 nitrite reduction, electron donor pseudoazurin, pre-reduced cytochrome cd1 Paracoccus pantotrophus
6.2
-
 O2 reduction, electron donor cytochrome c550 Paracoccus pantotrophus
6.2
-
 O2 reduction, electron donor cytochrome c550, pre-reduced cytochrome cd1 Paracoccus pantotrophus
6.3
-
 O2 reduction, electron donor pseudoazurin Paracoccus pantotrophus
6.3
-
 O2 reduction, electron donor pseudoazurin, pre-reduced cytochrome cd1 Paracoccus pantotrophus

Cofactor

Cofactor Comment Organism Structure
heme c
-
 Paracoccus pantotrophus
Heme d1
-
 Paracoccus pantotrophus