Cloned (Comment) | Organism |
---|---|
expression of wild-type and Y10F mutant enzyme in Pseudomonas putida | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
Y10F | no change in optical spectrum, nitrite and oxidase activity and heme c to heme d1 electron transfer rates compared to wild-type | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00084 | - |
azurin | recombinant Y10F mutant enzyme, at pH 6.2 and 27°C | Pseudomonas aeruginosa | |
0.00177 | - |
ferricytochrome c551 | recombinant wild-type enzyme, at pH 6.2 and 27°C | Pseudomonas aeruginosa | |
0.035 | - |
azurin | recombinant wild-type and Y10F mutant enzyme, at pH 6.2 and 27°C | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and Y10F mutant enzyme | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitric oxide + H2O + ferricytochrome c551 | - |
Pseudomonas aeruginosa | nitrite + ferrocytochrome c551 + H+ | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.583 | - |
ferricytochrome c551 | recombinant wild-type enzyme, at pH 6.2 and 27°C | Pseudomonas aeruginosa | |
0.6 | - |
ferricytochrome c551 | recombinant Y10F mutant enzyme, at pH 6.2 and 27°C | Pseudomonas aeruginosa | |
1 | - |
azurin | recombinant Y10F mutant enzyme, at pH 6.2 and 27°C | Pseudomonas aeruginosa | |
1.12 | - |
azurin | recombinant wild-type enzyme, at pH 6.2 and 27°C | Pseudomonas aeruginosa |