Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Subtilisin | species of 48000 Da which contains the d1 but not the c heme | Pseudomonas aeruginosa |
Cloned (Comment) | Organism |
---|---|
epression in Pseudomonas putida | Pseudomonas aeruginosa |
General Stability | Organism |
---|---|
urea, 4 M, 9% of initial activity, 6 M, no dissociation into subunits but irreversible inactivation | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
O2 | - |
Pseudomonas aeruginosa | |
0.0305 | - |
ferricytochrome c | succinylated monomeric enzyme | Pseudomonas aeruginosa | |
0.053 | - |
NO2- | - |
Pseudomonas aeruginosa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Paracoccus denitrificans | - |
- |
periplasm | - |
Pseudomonas aeruginosa | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60204 | - |
2 * 60204, deduced from amino acid sequence | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ferrocytochrome c-551 + O2 | Pseudomonas aeruginosa | - |
ferricytochrome c-551 + H2O | - |
? | |
NO2- + ferrocytochrome c | Pseudomonas aeruginosa | probably most dominant activity in vivo | NO + ferricytochrome c | - |
? | |
reduced azurin + O2 | Pseudomonas aeruginosa | not known whether azurin donates electrons in vivo in parallel or sequentially to cytochrome c551 | oxidized azurin + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alcaligenes faecalis | - |
- |
- |
Halomonas halodenitrificans | - |
- |
- |
Paracoccus denitrificans | - |
- |
- |
Paracoccus pantotrophus | - |
- |
- |
Pseudomonas aeruginosa | - |
- |
- |
Pseudomonas stutzeri | - |
- |
- |
Thiobacillus denitrificans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Alcaligenes faecalis |
- |
Pseudomonas stutzeri |
- |
Paracoccus pantotrophus |
- |
Halomonas halodenitrificans |
monomeric enzyme prepared by controlled succinylation of the native dimer | Pseudomonas aeruginosa |
recombinant enzyme, contains only the c heme | Pseudomonas aeruginosa |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
nitric oxide + H2O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H+ | proposed reaction mechanism | Pseudomonas aeruginosa |
Renatured (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ferrocytochrome c-551 + NO2- | - |
Paracoccus denitrificans | NO + ferricytochrome c-551 | - |
? | |
ferrocytochrome c-551 + NO2- | - |
Pseudomonas aeruginosa | NO + ferricytochrome c-551 | - |
? | |
ferrocytochrome c-551 + O2 | - |
Paracoccus denitrificans | H2O + ferricytochrome c-551 | - |
? | |
ferrocytochrome c-551 + O2 | inactive with eukaryotic cytochromes c | Pseudomonas aeruginosa | H2O + ferricytochrome c-551 | - |
? | |
ferrocytochrome c-551 + O2 | - |
Pseudomonas aeruginosa | ferricytochrome c-551 + H2O | - |
? | |
nitric oxide + H2O + ferricytochrome c | - |
Pseudomonas aeruginosa | nitrite + ferrocytochrome c + H+ | - |
r | |
NO2- + ferrocytochrome c | probably most dominant activity in vivo | Pseudomonas aeruginosa | NO + ferricytochrome c | - |
? | |
reduced azurin + O2 | not known whether azurin donates electrons in vivo in parallel or sequentially to cytochrome c551 | Pseudomonas aeruginosa | oxidized azurin + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Pseudomonas stutzeri |
dimer | - |
Thiobacillus denitrificans |
dimer | 2 * 60204, deduced from amino acid sequence | Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.33 | - |
ferricytochrome c | succinylated monomeric enzyme | Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme c | - |
Pseudomonas stutzeri | |
heme c | c heme is the electron accepting pole of the enzyme | Pseudomonas aeruginosa |