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Literature summary for 1.7.1.7 extracted from

  • Brox, L.W.; Hampton, A.
    Inactivation of guanosine 5-phosphate reductase by 6-chloro-, 6-mercapto-, and 2-amino-6-mercapto-9-beta-D-ribofuranosylpurine 5-phosphates (1968), Biochemistry, 7, 398-405.
    View publication on PubMed
Search for more literature for 1.7.1.7

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 2 mM required for maximal activity Klebsiella aerogenes
cysteine 2 mM required for maximal activity Klebsiella aerogenes
GSH 2 mM required for maximal activity Klebsiella aerogenes

Inhibitors

Inhibitors Comment Organism Structure
6-Chloro-9-beta-D-ribofuranosylpurine 5'-phosphate 0.01 mM, 80% inhibition after 40 min, 1 mM GMP protect Klebsiella aerogenes
6-Thio-GMP 0.009 mM, 81% inhibition after 30 min Klebsiella aerogenes
6-thio-IMP 0.32 mM, 75% inhibition after 15 min Klebsiella aerogenes
adenosine 2'-phosphate
-
 Klebsiella aerogenes
ADP 2 mM, 85% inhibition Klebsiella aerogenes
AMP
-
 Klebsiella aerogenes
ATP 0.4 mM, 92% inhibition Klebsiella aerogenes
GTP slight Klebsiella aerogenes
IMP
-
 Klebsiella aerogenes
iodoacetamide progressive inhibition, 2 mM GMP protect Klebsiella aerogenes
iodoacetate progressive inhibition, 2 mM GMP protect Klebsiella aerogenes
p-mercuribenzoate 2 mM, complete inhibition, complete protection by 2 mM GSH Klebsiella aerogenes

Organism

Organism UniProt Comment Textmining
Klebsiella aerogenes
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Klebsiella aerogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 212.0 units/mg, 1 unit is defined as the amount of enzyme giving an optical density change of 1.00/min Klebsiella aerogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADPH + guanosine 5'-phosphate
-
 Klebsiella aerogenes NADP+ + inosine 5'-phosphate + NH3
-
 ?