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Literature summary for 1.7.1.7 extracted from

  • Mackenzie, J.J.; Sorensen, L.B.
    Guanosine 5-phosphate reductase of human erythrocytes (1973), Biochim. Biophys. Acta, 327, 282-294.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol activation Homo sapiens
dithiothreitol activation Homo sapiens
thioglycolic acid activation Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate) 0.66 mM, 33% inhibition after 30 min Homo sapiens
Ca2+ 10 mM, 54% inhibition Homo sapiens
Cu2+
-
Homo sapiens
Fe2+ 10 mM, more than 90% inhibition Homo sapiens
IMP
-
Homo sapiens
Mg2+ 10 mM, more than 90% inhibition, 1 mM, 12% inhibition Homo sapiens
Mn2+
-
Homo sapiens
Ni2+
-
Homo sapiens
p-hydroxymercuribenzoate 0.66 mM, 18% inhibition after 30 min Homo sapiens
XMP
-
Homo sapiens
Zn2+ 10 mM, more than 90% inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0049
-
GMP
-
Homo sapiens
0.0085
-
NADPH
-
Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Reaction

Reaction Comment Organism Reaction ID
IMP + NH3 + NADP+ = GMP + NADPH + H+ first order reaction Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0052
-
-
Homo sapiens

Storage Stability

Storage Stability Organism
-70°C, 2 months, less than 20% loss of activity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADPH + guanosine 5'-phosphate
-
Homo sapiens NADP+ + inosine 5'-phosphate + NH3
-
ir

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
67
-
15 min, 40% loss of activity Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6 9 pH 6: about 50% of activity at maximum, pH 9: about 70% of activity at maximum Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0011
-
XMP
-
Homo sapiens