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Literature summary for 1.7.1.7 extracted from
- Reaction mechanism and specificity of human GMP reductase. Substrates, inhibitors, activators, and inactivators (1979), J. Biol. Chem., 254, 2308-2315.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| GTP | nonessential activator | Homo sapiens |  |
General Stability
| General Stability | Organism |
|---|---|
| 2-mercaptoethanol stabilizes | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2'-dXMP | - |
Homo sapiens | |
| 6-Chloropurine ribonucleotide | - |
Homo sapiens | |
| 6-Thio-GMP | - |
Homo sapiens | |
| 6-thio-IMP | - |
Homo sapiens | |
| 6-Thio-XMP | - |
Homo sapiens | |
| 8-Aza-7-deaza-XMP | - |
Homo sapiens | |
| 8-Aza-XMP | - |
Homo sapiens | |
| arabinosyl-XMP | - |
Homo sapiens | |
| XMP | competitive vs. GMP, noncompetitive vs. NADPH | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0026 | - |
GMP | - |
Homo sapiens | |
| 0.0075 | - |
GMP | data from the low concentration range | Homo sapiens | |
| 0.0169 | - |
NADPH | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 170000 | - |
gel filtration | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| affinity purification on Cibacron blue | Homo sapiens |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| IMP + NH3 + NADP+ = GMP + NADPH + H+ | mechanism: ordered sequential with GMP binding first | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| erythrocyte | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.35 | - |
- |
Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, 2-mercaptoethanol, 6 months, less than 15% loss of activity after several rounds of freezing and thawing | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NADPH + guanosine 5'-phosphate | less than 10% of the NADPH rate: thionicotinamide-NADPH, deamino-NADPH, 3-acetylpyrimidine-NADPH | Homo sapiens | NADP+ + inosine 5'-phosphate + NH3 | - |
? | |
| NADPH + guanosine 5'-phosphate | arabinosylGMP, 2'-dGMP and 8-azaGMP are reductively deaminated to their corresponding IMP analog at rates 1-2% the rate with GMP | Homo sapiens | NADP+ + inosine 5'-phosphate + NH3 | - |
? | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | no activity with NADH | Homo sapiens | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00017 | - |
XMP | - |
Homo sapiens | |
| 0.0003 | - |
8-Aza-XMP | - |
Homo sapiens | |
| 0.026 | - |
2'-dXMP | - |
Homo sapiens | |
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