Application | Comment | Organism |
---|---|---|
environmental protection | Geobacter sulfurreducens useful for the decontamination of environments polluted with azo dyes. The contribution of extracellular respiration to pollutants reduction will broaden the environmental applications | Geobacter sulfurreducens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion of genes omcB, omcC, and omcE encoding outer membrane proteins of Geobacter sulfurrescens modestly inhibit the decolorization activity by 16%, 42%, and 35%, respectively | Geobacter sulfurreducens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Geobacter sulfurreducens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Geobacter sulfurreducens | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-(dimethylamino)azobenzene + NADPH + H+ | Geobacter sulfurreducens | - |
N,N-dimethyl-1,4-phenylenediamine + aniline + NADP+ | - |
? | |
4-(dimethylamino)azobenzene + NADPH + H+ | Geobacter sulfurreducens PCA | - |
N,N-dimethyl-1,4-phenylenediamine + aniline + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacter sulfurreducens | - |
- |
- |
Geobacter sulfurreducens PCA | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-(dimethylamino)azobenzene + NADPH + H+ | - |
Geobacter sulfurreducens | N,N-dimethyl-1,4-phenylenediamine + aniline + NADP+ | - |
? | |
4-(dimethylamino)azobenzene + NADPH + H+ | - |
Geobacter sulfurreducens PCA | N,N-dimethyl-1,4-phenylenediamine + aniline + NADP+ | - |
? | |
4-([4-(dimethylamino)phenyl]diazenyl)benzene-1-sulfonate + NADPH + H+ | i.e. methyl orange | Geobacter sulfurreducens | ? + NADP+ | - |
? | |
4-([4-(dimethylamino)phenyl]diazenyl)benzene-1-sulfonate + NADPH + H+ | i.e. methyl orange | Geobacter sulfurreducens PCA | ? + NADP+ | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Geobacter sulfurreducens |
General Information | Comment | Organism |
---|---|---|
metabolism | essential role of exclusively extracellular respiration in azo dye decolorization. Electrons from acetate oxidization by this strain are transferred by the respiratory chain to methyl orange. As Geobacter sulfurreducens strain PCA does not express known azo reductases, extracellular electron transfer is an alternative way to decolor methyl orange. Outermembrane cytochromes are reported to play a prominent role in electron acceptor in strain PCS. The outermembrane proteins, OmcB, OmcC, and OmcE, are identified as the key proteins in extracellular reduction by the electrochemically active bacterium | Geobacter sulfurreducens |
additional information | deletion of genes omcB, omcC, and omcE encoding outer membrane proteins of Geobacter sulfurrescens modestly inhibit the decolorization activity by 16%, 42%, and 35%, respectively | Geobacter sulfurreducens |
physiological function | the enzyme catalyzes extracellular bioreduction of methyl orange. Geobacter sulfurreducens strain PCA has a very high decolorization efficiency among the methyl orange reducing bacteria | Geobacter sulfurreducens |