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Literature summary for 1.7.1.6 extracted from
- Identification of Enterococcus faecalis enzymes with azoreductases and/or nitroreductase activity (2017), BMC Microbiol., 17, 126 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene EF_0404, phylogenetic analysis and tree, recombinant overexpression of His-tagged enzyme in Escherichia coli strain XL1-Blue from vector pQE30 | Enterococcus faecalis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Enterococcus faecalis | Q838N5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant FMN cofactor-bound His-tagged enzyme from Escherichia coli strain XL1-Blue by affinity chromatography and dialysis | Enterococcus faecalis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-dimethylaminoazobenzene-2'-carboxylic acid + 2 NADPH + 2 H+ | i.e. methyl red | Enterococcus faecalis | anthranilate + N,N'-dimethylamino-aniline + 2 NADP+ | - |
? |  |
| additional information | the enzyme can also catalyze the nitroreduction of 7-nitrocoumarin-3-carboxylic acid in a FMN-dependent manner | Enterococcus faecalis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 25000, recombinant His-tagged enzyme, SDS-PAGE | Enterococcus faecalis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| azoreductase | - |
Enterococcus faecalis |
| EF0404 | - |
Enterococcus faecalis |
| EF_0404 | - |
Enterococcus faecalis |
| FMN-dependent NAD(P)H nitroreductase | - |
Enterococcus faecalis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
assay at | Enterococcus faecalis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 59 | - |
Tm of gene EF_0404 | Enterococcus faecalis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Enterococcus faecalis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | enzyme-bound prosthetic group, FMN-dependence for azoreductase activity | Enterococcus faecalis | |
| additional information | the enzyme can use both cofactors, NADH and NADPH, it shows a preference towards NADH for the azoreductase activity, but a preference towards NADPH for the nitroreductase activity | Enterococcus faecalis | |
| NADH | - |
Enterococcus faecalis | |
| NADPH | - |
Enterococcus faecalis | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | enzyme EF0404 shows both nitroreductase and azoreductase activity. The biochemical characteristics, such as substrate and cofactor specificity, of enzyme EF0404 resemble the properties of the known azoreductase AzoA. But its sequence matches with the nitroreductase group B, the same as enzyme EF0648 | Enterococcus faecalis |
| physiological function | enzyme EF0404 shows both nitroreductase and azoreductase activity. The biochemical characteristics, such as substrate and cofactor specificity, of enzyme EF0404 resemble the properties of the known azoreductase AzoA. But its sequence matches with the nitroreductase group, the same as enzyme EF0648 | Enterococcus faecalis |
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