KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
naphthol blue black | pH 7.0, 30°C | Pseudomonas sp. B1 | |
0.09 | - |
NADH | pH 7.0, 30°C | Pseudomonas sp. B1 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Pseudomonas sp. B1 | 5622 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
native PAGE | Pseudomonas sp. B1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. B1 | - |
- |
- |
Oxidation Stability | Organism |
---|---|
oxygen insensitive intracellular azoreductase | Pseudomonas sp. B1 |
Purification (Comment) | Organism |
---|---|
native intracellular enzyme 94.2fold from mutant strain B1 by ammonium sulfate fracionation and anion exchange chromatography | Pseudomonas sp. B1 |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | maximal enzyme production of 0.39 U/ml in mutant strain B1 at pH 6.5, 35°C, 2% D-glucose, 1% sodium nitrate, and 2% of inoculum concentration | Pseudomonas sp. B1 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
19.8 | - |
purified native enzyme, pH 7.0, 30°C | Pseudomonas sp. B1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
naphthol blue black + NADH + H+ | 78% conversion, analysis by UV-visible and FITR spectroscopy, HPLC, and GC-MS | Pseudomonas sp. B1 | ? + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 29000, SDS-PAGE | Pseudomonas sp. B1 |
Synonyms | Comment | Organism |
---|---|---|
azoreductase | - |
Pseudomonas sp. B1 |
oxygen insensitive intracellular azoreductase | - |
Pseudomonas sp. B1 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Pseudomonas sp. B1 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 50 | activity range, profile overview | Pseudomonas sp. B1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Pseudomonas sp. B1 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | activity range, profile overview | Pseudomonas sp. B1 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Pseudomonas sp. B1 |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme degrades the azo dye naphthol blue black, the reaction product is less toxic than the substrate | Pseudomonas sp. B1 |