Literature summary for 1.7.1.6 extracted from

Matsumoto, K.; Mukai, Y.; Ogata, D.; Shozui, F.; Nduko, J.; Taguchi, S.; Ooi, T.
Characterization of thermostable FMN-dependent NADH azoreductase from the moderate thermophile Geobacillus stearothermophilus (2010), Appl. Microbiol. Biotechnol., 86, 1431-1438.

Search for more literature for 1.7.1.6

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
23600	-	2 * 23600, SDS-PAGE	Geobacillus stearothermophilus
46000	-	gel filtration	Geobacillus stearothermophilus

Organism

Organism	UniProt	Comment	Textmining
Geobacillus stearothermophilus	Q9X4K2	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.2	-	cosubstrate NADPH, pH 7.4, 30°C	Geobacillus stearothermophilus
25.8	-	cosubstrate NADH, pH 7.4, 30°C	Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1-(2-pyridylazo)-2-naphthol + NADH + H+	-	Geobacillus stearothermophilus	? + NAD+	-	ir
acid red 88 + NADH + H+	10% of hte activity with methyl red at 30°C	Geobacillus stearothermophilus	? + NAD+	-	ir
Congo red + NADH + H+	-	Geobacillus stearothermophilus	? + NAD+	-	ir
ethyl red + NADH + H+	best substrate, 182% of the activity with methyl red at 30°C	Geobacillus stearothermophilus	? + NAD+	-	ir
methyl red + 2 NADH + 2 H+	-	Geobacillus stearothermophilus	o-aminobenzoic acid + N,N-dimethyl-p-phenylenediamine + 2 NAD+	-	ir
orange I + NADH + H+	-	Geobacillus stearothermophilus	? + NAD+	-	ir

Subunits

Subunits	Comment	Organism
dimer	2 * 23600, SDS-PAGE	Geobacillus stearothermophilus

Synonyms

Synonyms	Comment	Organism
AzrG	-	Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
85	-	-	Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
30	-	one month, 67% residual activity	Geobacillus stearothermophilus
60	-	10 min, no loss of activity	Geobacillus stearothermophilus

Cofactor

Cofactor	Comment	Organism	Structure
FMN	-	Geobacillus stearothermophilus
additional information	no substrates: NADP+, NAD+	Geobacillus stearothermophilus
NADH	NADH preferred over NADPH	Geobacillus stearothermophilus
NADPH	NADH is preferred over NADPH	Geobacillus stearothermophilus

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Release 2023.1 (January 2023)