Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Enterococcus faecium |
into the pCR2.1-TOPO vector and subsequently into pET15b for expression in Escherichia coli NovaBlue DE3 cells | Enterococcus faecium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0164 | - |
Methyl red | - |
Enterococcus faecium | |
0.0164 | - |
Methyl red | pH 7.0, 25°C | Enterococcus faecium | |
0.171 | - |
NADH | pH 7.0, 25°C | Enterococcus faecium | |
0.1714 | - |
NADH | - |
Enterococcus faecium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
determined by SDS-PAGE | Enterococcus faecium |
23000 | - |
x * 23000, SDS-PAGE | Enterococcus faecium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecium | - |
- |
- |
Enterococcus faecium | D0EFL2 | - |
- |
Enterococcus faecium ATCC 6569 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
via the hexa-histidine tag | Enterococcus faecium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.575 | - |
substrate direct Blue 15, cofactor NADPH | Enterococcus faecium |
3.58 | - |
substrate direct blue 15, cosubstrate NADPH, pH 7.0, 25°C | Enterococcus faecium |
3.699 | - |
substrate direct Blue 15, cofactor NADH | Enterococcus faecium |
3.7 | - |
substrate direct blue 15, cosubstrate NADH, pH 7.0, 25°C | Enterococcus faecium |
17 | - |
substrate Congo red, cosubstrate NADPH, pH 7.0, 25°C | Enterococcus faecium |
17.02 | - |
substrate congo red, cofactor NADPH | Enterococcus faecium |
18.18 | - |
substrate orange II, cofactor NADH | Enterococcus faecium |
18.2 | - |
substrate orange II, cosubstrate NADH, pH 7.0, 25°C | Enterococcus faecium |
20.7 | - |
substrate Ponceau BS, cofactor NADH | Enterococcus faecium |
20.7 | - |
substrate ponceau BS, cosubstrate NADH, pH 7.0, 25°C | Enterococcus faecium |
23.2 | - |
substrate acid red 88, cosubstrate NADPH, pH 7.0, 25°C | Enterococcus faecium |
23.23 | - |
substrate acid red 88, cofactor NADPH | Enterococcus faecium |
24.29 | - |
substrate methyl red, cofactor NADPH | Enterococcus faecium |
24.3 | - |
substrate methyl red, cosubstrate NADPH, pH 7.0, 25°C | Enterococcus faecium |
76.6 | - |
substrate acid red 88, cosubstrate NADH, pH 7.0, 25°C | Enterococcus faecium |
76.63 | - |
substrate acid red 88, cofactor NADH | Enterococcus faecium |
682 | - |
substrate Congo red, cosubstrate NADH, pH 7.0, 25°C | Enterococcus faecium |
682.2 | - |
substrate congo red, cofactor NADH | Enterococcus faecium |
5095 | - |
substrate methyl red, cofactor NADH | Enterococcus faecium |
5095 | - |
substrate methyl red, cosubstrate NADH, pH 7.0, 25°C | Enterococcus faecium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acid red 88 + NAD(P)H + H+ | - |
Enterococcus faecium | ? | - |
? | |
acid red 88 + NADH + H+ | - |
Enterococcus faecium | ? + NAD+ | - |
? | |
acid red 88 + NADH + H+ | - |
Enterococcus faecium ATCC 6569 | ? + NAD+ | - |
? | |
congo red + NAD(P)H + H+ | - |
Enterococcus faecium | ? | - |
? | |
Congo red + NADH + H+ | - |
Enterococcus faecium | ? + NAD+ | - |
? | |
Congo red + NADH + H+ | - |
Enterococcus faecium ATCC 6569 | ? + NAD+ | - |
? | |
direct blue 15 + NAD(P)H + H+ | - |
Enterococcus faecium | ? | - |
? | |
direct blue 15 + NADH + H+ | - |
Enterococcus faecium | ? + NAD+ | - |
? | |
direct blue 15 + NADH + H+ | - |
Enterococcus faecium ATCC 6569 | ? + NAD+ | - |
? | |
methyl red + 2 NADH + 2 H+ | - |
Enterococcus faecium | anthranilate + N,N-dimethyl-1,4-phenylenediamine + 2 NAD+ | - |
r | |
methyl red + NAD(P)H + H+ | - |
Enterococcus faecium | ? | - |
? | |
methyl red + NADH + H+ | - |
Enterococcus faecium | ? + NAD+ | - |
? | |
methyl red + NADH + H+ | - |
Enterococcus faecium ATCC 6569 | ? + NAD+ | - |
? | |
methyl red + NADPH + H+ | - |
Enterococcus faecium | ? + NADP+ | - |
? | |
additional information | no substrates: ponceau S, tartrazine, amaranth | Enterococcus faecium | ? | - |
? | |
additional information | no substrates: ponceau S, tartrazine, amaranth | Enterococcus faecium ATCC 6569 | ? | - |
? | |
orange II + NAD(P)H + H+ | - |
Enterococcus faecium | ? | - |
? | |
orange II + NADH + H+ | - |
Enterococcus faecium | ? + NAD+ | - |
? | |
Ponceau BS + NAD(P)H + H+ | - |
Enterococcus faecium | ? | - |
? | |
ponceau BS + NADH + H+ | - |
Enterococcus faecium | ? + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 23000, SDS-PAGE | Enterococcus faecium |
Synonyms | Comment | Organism |
---|---|---|
acpD | - |
Enterococcus faecium |
AzoEF1 | - |
Enterococcus faecium |
azoreductase | - |
Enterococcus faecium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
activity assay at room temperature | Enterococcus faecium |
25 | - |
assay at | Enterococcus faecium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10 | - |
NADH | - |
Enterococcus faecium | |
10 | - |
NADH | pH 7.0, 25°C | Enterococcus faecium | |
17 | - |
Methyl red | - |
Enterococcus faecium | |
17 | - |
Methyl red | pH 7.0, 25°C | Enterococcus faecium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Enterococcus faecium |
7 | - |
activity assay | Enterococcus faecium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Enterococcus faecium | |
NADH | - |
Enterococcus faecium | |
NADH | preferred over NADPH | Enterococcus faecium | |
NADPH | - |
Enterococcus faecium |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
60 | - |
NADH | pH 7.0, 25°C | Enterococcus faecium | |
1000 | - |
Methyl red | pH 7.0, 25°C | Enterococcus faecium |