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Literature summary for 1.7.1.6 extracted from

  • Macwana, S.R.; Punj, S.; Cooper, J.; Schwenk, E.; John, G.H.
    Identification and Isolation of an Azoreductase from Enterococcus faecium (2009), Curr. Issues Mol. Biol., 12, 43-48.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Enterococcus faecium
into the pCR2.1-TOPO vector and subsequently into pET15b for expression in Escherichia coli NovaBlue DE3 cells Enterococcus faecium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0164
-
Methyl red
-
Enterococcus faecium
0.0164
-
Methyl red pH 7.0, 25°C Enterococcus faecium
0.171
-
NADH pH 7.0, 25°C Enterococcus faecium
0.1714
-
NADH
-
Enterococcus faecium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
determined by SDS-PAGE Enterococcus faecium
23000
-
x * 23000, SDS-PAGE Enterococcus faecium

Organism

Organism UniProt Comment Textmining
Enterococcus faecium
-
-
-
Enterococcus faecium D0EFL2
-
-
Enterococcus faecium ATCC 6569
-
-
-

Purification (Commentary)

Purification (Comment) Organism
via the hexa-histidine tag Enterococcus faecium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.575
-
substrate direct Blue 15, cofactor NADPH Enterococcus faecium
3.58
-
substrate direct blue 15, cosubstrate NADPH, pH 7.0, 25°C Enterococcus faecium
3.699
-
substrate direct Blue 15, cofactor NADH Enterococcus faecium
3.7
-
substrate direct blue 15, cosubstrate NADH, pH 7.0, 25°C Enterococcus faecium
17
-
substrate Congo red, cosubstrate NADPH, pH 7.0, 25°C Enterococcus faecium
17.02
-
substrate congo red, cofactor NADPH Enterococcus faecium
18.18
-
substrate orange II, cofactor NADH Enterococcus faecium
18.2
-
substrate orange II, cosubstrate NADH, pH 7.0, 25°C Enterococcus faecium
20.7
-
substrate Ponceau BS, cofactor NADH Enterococcus faecium
20.7
-
substrate ponceau BS, cosubstrate NADH, pH 7.0, 25°C Enterococcus faecium
23.2
-
substrate acid red 88, cosubstrate NADPH, pH 7.0, 25°C Enterococcus faecium
23.23
-
substrate acid red 88, cofactor NADPH Enterococcus faecium
24.29
-
substrate methyl red, cofactor NADPH Enterococcus faecium
24.3
-
substrate methyl red, cosubstrate NADPH, pH 7.0, 25°C Enterococcus faecium
76.6
-
substrate acid red 88, cosubstrate NADH, pH 7.0, 25°C Enterococcus faecium
76.63
-
substrate acid red 88, cofactor NADH Enterococcus faecium
682
-
substrate Congo red, cosubstrate NADH, pH 7.0, 25°C Enterococcus faecium
682.2
-
substrate congo red, cofactor NADH Enterococcus faecium
5095
-
substrate methyl red, cofactor NADH Enterococcus faecium
5095
-
substrate methyl red, cosubstrate NADH, pH 7.0, 25°C Enterococcus faecium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acid red 88 + NAD(P)H + H+
-
Enterococcus faecium ?
-
?
acid red 88 + NADH + H+
-
Enterococcus faecium ? + NAD+
-
?
acid red 88 + NADH + H+
-
Enterococcus faecium ATCC 6569 ? + NAD+
-
?
congo red + NAD(P)H + H+
-
Enterococcus faecium ?
-
?
Congo red + NADH + H+
-
Enterococcus faecium ? + NAD+
-
?
Congo red + NADH + H+
-
Enterococcus faecium ATCC 6569 ? + NAD+
-
?
direct blue 15 + NAD(P)H + H+
-
Enterococcus faecium ?
-
?
direct blue 15 + NADH + H+
-
Enterococcus faecium ? + NAD+
-
?
direct blue 15 + NADH + H+
-
Enterococcus faecium ATCC 6569 ? + NAD+
-
?
methyl red + 2 NADH + 2 H+
-
Enterococcus faecium anthranilate + N,N-dimethyl-1,4-phenylenediamine + 2 NAD+
-
r
methyl red + NAD(P)H + H+
-
Enterococcus faecium ?
-
?
methyl red + NADH + H+
-
Enterococcus faecium ? + NAD+
-
?
methyl red + NADH + H+
-
Enterococcus faecium ATCC 6569 ? + NAD+
-
?
methyl red + NADPH + H+
-
Enterococcus faecium ? + NADP+
-
?
additional information no substrates: ponceau S, tartrazine, amaranth Enterococcus faecium ?
-
?
additional information no substrates: ponceau S, tartrazine, amaranth Enterococcus faecium ATCC 6569 ?
-
?
orange II + NAD(P)H + H+
-
Enterococcus faecium ?
-
?
orange II + NADH + H+
-
Enterococcus faecium ? + NAD+
-
?
Ponceau BS + NAD(P)H + H+
-
Enterococcus faecium ?
-
?
ponceau BS + NADH + H+
-
Enterococcus faecium ? + NAD+
-
?

Subunits

Subunits Comment Organism
? x * 23000, SDS-PAGE Enterococcus faecium

Synonyms

Synonyms Comment Organism
acpD
-
Enterococcus faecium
AzoEF1
-
Enterococcus faecium
azoreductase
-
Enterococcus faecium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
activity assay at room temperature Enterococcus faecium
25
-
assay at Enterococcus faecium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
10
-
NADH
-
Enterococcus faecium
10
-
NADH pH 7.0, 25°C Enterococcus faecium
17
-
Methyl red
-
Enterococcus faecium
17
-
Methyl red pH 7.0, 25°C Enterococcus faecium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
Enterococcus faecium
7
-
activity assay Enterococcus faecium

Cofactor

Cofactor Comment Organism Structure
FMN
-
Enterococcus faecium
NADH
-
Enterococcus faecium
NADH preferred over NADPH Enterococcus faecium
NADPH
-
Enterococcus faecium

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
60
-
NADH pH 7.0, 25°C Enterococcus faecium
1000
-
Methyl red pH 7.0, 25°C Enterococcus faecium