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Literature summary for 1.7.1.6 extracted from
- Decolorizing kinetics of a recombinant Escherichia coli SS125 strain harboring azoreductase gene from Bacillus latrosporus RRK1 (2008), Biores. Technol., 99, 2187-2191.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the vector pET32a for recombinant expression in Escherichia coli DH5alpha cells | Brevibacillus laterosporus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
determined by SDS-PAGE | Brevibacillus laterosporus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brevibacillus laterosporus | - |
- |
- |
| Brevibacillus laterosporus RRK1 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| a Cibacron blue agarose 3G column is used | Brevibacillus laterosporus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.8 | - |
crude cell extract | Brevibacillus laterosporus |
| 505 | - |
purification step Cibacron blue agarose 3G | Brevibacillus laterosporus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| golden yellow + NADH + H+ | - |
Brevibacillus laterosporus | ? | - |
? |  |
| golden yellow + NADH + H+ | - |
Brevibacillus laterosporus RRK1 | ? | - |
? | |
| red RB + NADH + H+ | - |
Brevibacillus laterosporus | ? | - |
? | |
| red RB + NADH + H+ | - |
Brevibacillus laterosporus RRK1 | ? | - |
? | |
| remazol orange + NADH + H+ | - |
Brevibacillus laterosporus | ? | - |
? | |
| remazol orange + NADH + H+ | - |
Brevibacillus laterosporus RRK1 | ? | - |
? | |
| remazol red + NADH + H+ | - |
Brevibacillus laterosporus | ? | - |
? | |
| remazol red + NADH + H+ | - |
Brevibacillus laterosporus RRK1 | ? | - |
? | |
| remazol yellow + NADH + H+ | - |
Brevibacillus laterosporus | ? | - |
? | |
| remazol yellow + NADH + H+ | - |
Brevibacillus laterosporus RRK1 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| azoreductase | - |
Brevibacillus laterosporus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
in gel activity assay | Brevibacillus laterosporus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Brevibacillus laterosporus | |
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Release 2023.1 (January 2023)
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