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Literature summary for 1.7.1.6 extracted from
- Enhancing survival of Escherichia coli by expression of azoreductase AZR possessing quinone reductase activity (2008), Appl. Microbiol. Biotechnol., 80, 409-416.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the vector pGEX 4T-1 for transformation into Escherichia coli JM109 cells | Cereibacter sphaeroides |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dicoumarol | - |
Cereibacter sphaeroides |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0133 | - |
anthraquinone-2-sulfonate | - |
Cereibacter sphaeroides | |
| 0.0161 | - |
anthraquinone-2,6-disulfonate | - |
Cereibacter sphaeroides | |
| 0.0162 | - |
2-methyl-1,4-naphthoquinone | - |
Cereibacter sphaeroides | |
| 0.0346 | - |
2-Hydroxy-1,4-naphthoquinone | - |
Cereibacter sphaeroides | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cereibacter sphaeroides | Q8GKS3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cereibacter sphaeroides |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-hydroxy-1,4-naphthoquinone + NADPH + H+ | lawsone, quinone reductase activity | Cereibacter sphaeroides | ? | - |
? | |
| 2-methyl-1,4-naphthoquinone + NADPH + H+ | menadione, quinone reductase activity | Cereibacter sphaeroides | ? | - |
? | |
| anthraquinone-2,6-disulfonate + NADPH + H+ | quinone reductase activity | Cereibacter sphaeroides | ? | - |
? | |
| anthraquinone-2-sulfonate + NADPH + H+ | quinone reductase activity | Cereibacter sphaeroides | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| azoreductase | - |
Cereibacter sphaeroides |
| AZR | - |
Cereibacter sphaeroides |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
substrate 2-methyl-1,4-naphthoquinone, quinone reductase activity | Cereibacter sphaeroides |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 211.2 | - |
anthraquinone-2,6-disulfonate | - |
Cereibacter sphaeroides | |
| 218.4 | - |
anthraquinone-2-sulfonate | - |
Cereibacter sphaeroides | |
| 345 | - |
2-Hydroxy-1,4-naphthoquinone | - |
Cereibacter sphaeroides | |
| 3278 | - |
2-methyl-1,4-naphthoquinone | - |
Cereibacter sphaeroides | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
quinone reductase activity | Cereibacter sphaeroides |
| 8.5 | - |
substrate 2-methyl-1,4-naphthoquinone, quinone reductase activity | Cereibacter sphaeroides |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9 | maximal activity | Cereibacter sphaeroides |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Cereibacter sphaeroides | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0876 | - |
dicoumarol | - |
Cereibacter sphaeroides | |
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Release 2023.1 (January 2023)
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