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Literature summary for 1.7.1.6 extracted from
- Biochemical and molecular characterization of an azoreductase from Staphylococcus aureus, a tetrameric NADPH-dependent flavoprotein (2005), Microbiology, 151, 1433-1441.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Staphylococcus aureus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.057 | - |
2-[4-(dimethylamino)phenylazo]benzoic acid | in 25 mM potassium phosphate buffer (pH 7.1), 0.25 mM 2-[4-(dimethylamino)phenylazo]benzoic acid and 0.1 mM NADPH | Staphylococcus aureus |  |
| 0.074 | - |
NADPH | in 25 mM potassium phosphate buffer (pH 7.1), 0.25 mM azo dye and 0.1 mM NADPH | Staphylococcus aureus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 20000 | - |
SDS-PAGE | Staphylococcus aureus |
| 85000 | - |
gel filtration | Staphylococcus aureus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus aureus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HiPrep 16/10 Q XL anion-exchange column chromatography and HiLoad 26/60 Superdex 200 gel fltration | Staphylococcus aureus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.015 | - |
crude cell extract | Staphylococcus aureus |
| 0.14 | - |
after purification | Staphylococcus aureus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-[4-(dimethylamino)phenylazo]benzoic acid + NADPH | - |
Staphylococcus aureus | N,N-dimethyl-p-phenylenediamine + 2-aminobenzoic acid + NADP+ | - |
? | |
| Amaranth + NADPH | - |
Staphylococcus aureus | ? + NADP+ | - |
? | |
| additional information | does not react with NADH as electron donor | Staphylococcus aureus | ? | - |
? | |
| Orange II + NADPH | - |
Staphylococcus aureus | ? + NADP+ | - |
? | |
| Ponceau BS + NADPH | - |
Staphylococcus aureus | ? + NADP+ | - |
? | |
| Ponceau S + NADPH | - |
Staphylococcus aureus | ? + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 20000, SDS-PAGE | Staphylococcus aureus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Azo 1 | - |
Staphylococcus aureus |
| NADPH-flavin azoreductase | - |
Staphylococcus aureus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | four non-covalently bound FMN | Staphylococcus aureus | |
| NADPH | - |
Staphylococcus aureus | |
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