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Literature summary for 1.7.1.6 extracted from
- Molecular cloning, overexpression, purification, and characterization of an aerobic FMN-dependent azoreductase from Enterococcus faecalis (2004), Protein Expr. Purif., 34, 302-310.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21-Gold(DE3)pLysS | Enterococcus faecalis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.024 | - |
2-[4-(dimethylamino)phenylazo]benzoic acid | pH 7.1, 23°C | Enterococcus faecalis |  |
| 0.14 | - |
NADH | pH 7.1, 23°C | Enterococcus faecalis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | - |
2 * 23000, SDS-PAGE | Enterococcus faecalis |
| 43000 | - |
gel filtration | Enterococcus faecalis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Enterococcus faecalis | important for the conversion of azo dyes in the gastrointestinal tract | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Enterococcus faecalis | - |
strain ATCC 19433, predominant member of the human gastrointestinal microflora | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Enterococcus faecalis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 63.9 | - |
purified enzyme, pH 7.1, 23°C, with Methyl red and NADH as substrates | Enterococcus faecalis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-(4'-carboxyphenylazo)-2-naphthol + NADH | substrate is carboxy-Orange II | Enterococcus faecalis | 4-amino-1-benzoic acid + NAD+ + 1-amino-2-hydroxynaphthalene | - |
? | |
| 2-[4-(dimethylamino)phenylazo]benzoic acid + NADH | substrate is Methyl red | Enterococcus faecalis | 2-aminobenzoic acid + N,N-dimethyl-1,4-phenylenediamine + NAD+ | - |
? | |
| Amaranth + NADH | - |
Enterococcus faecalis | ? | - |
? | |
| additional information | important for the conversion of azo dyes in the gastrointestinal tract | Enterococcus faecalis | ? | - |
? | |
| Orange G + NADH | - |
Enterococcus faecalis | ? | - |
? | |
| Ponceau BS + NADH | - |
Enterococcus faecalis | ? | - |
? | |
| Ponceau S + NADH | - |
Enterococcus faecalis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 23000, SDS-PAGE | Enterococcus faecalis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | one molecule per enzyme dimer | Enterococcus faecalis | |
| NADH | - |
Enterococcus faecalis | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Enterococcus faecalis | calculated | - |
4.8 |
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